ZFIN ID: ZDB-PUB-170922-15
Muscle stem cells and regeneration in zebrafish models of muscular dystrophies
Hall, T.E., Bryson-Richardson, R.J., Cole, N.J., Currie, P.D.
The growth, repair and regeneration of skeletal muscle is dependent upon a
population of mononuclear precursor cells known as satellite cells.
Recently, the classical view of skeletal muscle satellite cells as a
homogeneous, monopotent population has been challenged by the
discovery of multipotent, muscle derived adult stem cells coexisting within
the myotome. Furthermore, several studies now suggest that satellite cells
comprise separate sub-populations exhibiting distinct biochemical and
biological properties. As such, many important questions are now posed as
to the normal role and function of these cellular compartments, as well as
their potential utility in cell-mediated therapies.
We are currently applying the power of the zebrafish genetic model to the
study of skeletal muscle stem cells for the first time, to answer questions
which have hitherto been difficult or impossible to address in other systems.
We are performing a comprehensive “cradle to grave” in situ hybridisation
and immunohistochemistry screen using zebrafish homologues of known
mammalian satellite cell (e.g. Pax7, syndecan 3/4, cmet) and stem cell
markers (e.g. Sca-1, c-kit, CD45). Expression patterns from wild-type fish
are also being compared with those from the sapje model of Duchenne’s
muscular dystrophy using a multi-dimensional scaling approach.
No data available