ZFIN ID: ZDB-PUB-170922-11
The first wave of T lymphopoiesis in zebrafish arises from aorta endothelium independent of hematopoietic stem cells
Tian, Y., Xu, J., Feng, S., He, S., Zhao, S., Zhu, L., Jin, W., Dai, Y., Luo, L., Qu, J.Y., Wen, Z.
Date: 2017
Source: The Journal of experimental medicine   214(11): 3347-3360 (Journal)
Registered Authors: Luo, Lingfei, Wen, Zilong
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Aorta/cytology*
  • Aorta/embryology
  • Aorta/metabolism
  • Embryo, Nonmammalian/cytology*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Endothelium, Vascular/cytology*
  • Endothelium, Vascular/embryology
  • Endothelium, Vascular/metabolism
  • Gene Expression Regulation, Developmental
  • Gonads/cytology
  • Gonads/embryology
  • Gonads/metabolism
  • Hematopoietic Stem Cells/cytology*
  • Hematopoietic Stem Cells/metabolism
  • In Situ Hybridization
  • Lymphopoiesis*
  • Mesonephros/cytology
  • Mesonephros/embryology
  • Mesonephros/metabolism
  • Microscopy, Confocal
  • T-Lymphocytes/cytology*
  • T-Lymphocytes/metabolism
  • Time-Lapse Imaging/methods
  • Zebrafish
PubMed: 28931624 Full text @ J. Exp. Med.
T lymphocytes are key cellular components of the adaptive immune system and play a central role in cell-mediated immunity in vertebrates. Despite their heterogeneities, it is believed that all different types of T lymphocytes are generated exclusively via the differentiation of hematopoietic stem cells (HSCs). Using temporal-spatial resolved fate-mapping analysis and time-lapse imaging, here we show that the ventral endothelium in the zebrafish aorta-gonad-mesonephros and posterior blood island, the hematopoietic tissues previously known to generate HSCs and erythromyeloid progenitors, respectively, gives rise to a transient wave of T lymphopoiesis independent of HSCs. This HSC-independent T lymphopoiesis occurs early and generates predominantly CD4 Tαβ cells in the larval but not juvenile and adult stages, whereas HSC-dependent T lymphopoiesis emerges late and produces various subtypes of T lymphocytes continuously from the larval stage to adulthood. Our study unveils the existence, origin, and ontogeny of HSC-independent T lymphopoiesis in vivo and reveals the complexity of the endothelial-hematopoietic transition of the aorta.