ZFIN ID: ZDB-PUB-170813-3
Activation of the Nkx2.5-Calr-p53 signaling pathway by hyperglycemia induces cardiac remodeling and dysfunction in adult zebrafish.
Yanyi, S., Qiuyun, W., Yuehua, F., Chunfang, W., Guoping, L., Zhenyue, C.
Date: 2017
Source: Disease models & mechanisms   10(10): 1217-1227 (Journal)
Registered Authors:
Keywords: Adult zebrafish, Apoptosis, Cardiomyopathy, Echocardiography, Hyperglycemia, Nkx2.5–Calr–p53
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Apoptosis
  • Blood Glucose/metabolism*
  • Calreticulin/genetics
  • Calreticulin/metabolism*
  • Cardiomegaly/genetics
  • Cardiomegaly/metabolism*
  • Cardiomegaly/pathology
  • Cardiomegaly/physiopathology
  • Cells, Cultured
  • Diabetic Cardiomyopathies/genetics
  • Diabetic Cardiomyopathies/metabolism*
  • Diabetic Cardiomyopathies/pathology
  • Diabetic Cardiomyopathies/physiopathology
  • Disease Models, Animal
  • Female
  • Gene Expression Regulation, Developmental
  • Homeobox Protein Nkx-2.5/genetics
  • Homeobox Protein Nkx-2.5/metabolism*
  • Hyperglycemia/blood
  • Hyperglycemia/genetics
  • Hyperglycemia/metabolism*
  • Myocytes, Cardiac/metabolism*
  • Myocytes, Cardiac/pathology
  • Signal Transduction
  • Time Factors
  • Tumor Suppressor Protein p53/genetics
  • Tumor Suppressor Protein p53/metabolism*
  • Ventricular Dysfunction/genetics
  • Ventricular Dysfunction/metabolism*
  • Ventricular Dysfunction/pathology
  • Ventricular Dysfunction/physiopathology
  • Ventricular Remodeling*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 28801532 Full text @ Dis. Model. Mech.
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ABSTRACT
Hyperglycemia is an independent risk factor for diabetic cardiomyopathy in humans; however, the underlying mechanisms have not been thoroughly elucidated. Zebrafish (Danio rerio) was used in this study as a novel vertebrate model to explore the signaling pathways of human adult cardiomyopathy. Hyperglycemia was induced by alternately immersing adult zebrafish in a glucose solution or water. The hyperglycemic fish gradually exhibited some hallmarks of cardiomyopathy such as myocardial hypertrophy and apoptosis, myofibril loss, fetal gene reactivation, and severe arrhythmia. Echocardiography of the glucose-treated fish demonstrated diastolic dysfunction at an early stage and systolic dysfunction at a later stage, consistent with what is observed in diabetic patients. Enlarged hearts with decreased myocardial density, accompanied by decompensated cardiac function, indicated that apoptosis was critical in the pathological process. Significant upregulation of the expression of Nkx2.5 and its downstream targets calreticulin (Calr) and p53 was noted in the glucose-treated fish. High-glucose stimulation in vitro evoked marked apoptosis of primary cardiomyocytes, which was rescued by the p53 inhibitor pifithrin-μ. In vitro experiments were performed using compound treatment and genetically via cell infection. Genetically, knockout of Nkx2.5 induced decreased expression of Nkx2.5, Calr and p53 Upregulation of Calr resulted in increased p53 expression, whereas the level of Nkx2.5 remained unchanged. An adult zebrafish model of hyperglycemia-induced cardiomyopathy was successfully established. Hyperglycemia-induced myocardial apoptosis was mediated, at least in part, by activation of the Nkx2.5-Calr-p53 pathway in vivo, resulting in cardiac dysfunction and hyperglycemia-induced cardiomyopathy.
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