PUBLICATION

Cloning, expression pattern and promoter functional analysis of cyp19a1a gene in miiuy croaker

Authors
Huang, W., Yang, P., Lv, Z., Wu, C., Gui, J., Lou, B.
ID
ZDB-PUB-170629-12
Date
2017
Source
Gene   627: 271-277 (Journal)
Registered Authors
Gui, Jian-Fang, Huang, Wei
Keywords
Miichthys miiuy, Promoter analysis, Vitellogenesis, cyp19a1a
MeSH Terms
  • Animals
  • Cloning, Molecular
  • Cytochrome P-450 CYP1A1/genetics*
  • Cytochrome P-450 CYP1A1/metabolism
  • Female
  • Fish Proteins/genetics*
  • Fish Proteins/metabolism
  • Gene Expression Regulation, Developmental*
  • Male
  • Ovary/growth & development
  • Ovary/metabolism
  • Perciformes/genetics
  • Perciformes/growth & development
  • Perciformes/metabolism*
  • Promoter Regions, Genetic*
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
28655540 Full text @ Gene
Abstract
Gonadal-specific aromatase encoded by cyp19a1a is the important enzyme controlling estrogen biosynthesis in teleosts. In the present study, the cDNA sequence of cyp19a1a was cloned and characterized from miiuy croaker Miichthys miiuy. The cDNA encoded a protein of 519 amino acids with five structural regions. Higher identities of amino acid sequences and conserved structural regions were found between Mmcyp19a1a and other cyp19a1a genes. In addition, Mmcyp19a1a was clustered together with other seawater fishes. Immunohistochemical analysis revealed that Mmcyp19a1a was localized exclusively in the cytoplasmic of thecal and granulosa cells surrounding the oocytes. Both the protein and mRNA levels of Mmcyp19a1a were increased significantly at the stage III follicles (mid-vitellogenic) and then decreased along with vitellogenesis. Interestingly, strong immunoreactive signals were also detected in the supporting cells of connective tissues during ovarian development. A 1777bp promoter fragment of Mmcyp19a1a was also isolated, and functional analysis using an EGFP reporter fusion in zebrafish larvae presented positive signals in the above of yolk sac, where is the region of pronephros and germ plasm occur. The Mmcyp19a1a:EGFP expression pattern was generally consistent with the endogenous cyp19a1a genesis. These results indicate that the Mmcyp19a1a gene plays an important role during vitellogenesis and oocyte maturation. The constructor of Mmcyp19a1a:EGFP may provide a useful tool for genetic analysis of gonad development in teleost.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping