PUBLICATION
Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
- Authors
- Wu, Y., Lou, Q.Y., Ge, F., Xiong, Q.
- ID
- ZDB-PUB-170624-11
- Date
- 2017
- Source
- Scientific Reports 7: 4022 (Journal)
- Registered Authors
- Keywords
- miRNAs, Proteomics
- MeSH Terms
-
- Animals
- Computational Biology/methods
- Embryo, Nonmammalian
- Embryonic Development/genetics*
- Female
- Gene Expression Regulation, Developmental*
- Gene Knockdown Techniques
- Male
- MicroRNAs*
- Proteomics*/methods
- RNA Interference
- RNA, Messenger/genetics
- Reproducibility of Results
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism*
- PubMed
- 28642470 Full text @ Sci. Rep.
Citation
Wu, Y., Lou, Q.Y., Ge, F., Xiong, Q. (2017) Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos. Scientific Reports. 7:4022.
Abstract
MicroRNAs (miRNAs) are noncoding RNAs which control gene expression by the suppression of translation or the degradation of mRNAs. Dre-miR-21 (miR-21) has been reported to impact cardiac valvulogenesis in zebrafish embryos. However, the target genes of miR-21 are still largely unknown. Here a tandem isobaric mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-21-regulated proteins. A total of 251 proteins were dysregulated after miR-21 knockdown, suggesting that they may be regulated by miR-21. Bioinformatics analysis indicated that these differentially expressed proteins (DEPs) participate in various biological processes, suggesting that miR-21 may be involved in diverse cellular pathways. Sixteen DEPs were also predicted to be miR-21 targets by at least two algorithms, and several candidate target genes were selected for further luciferase reporter analysis. The results showed that genes encoding tropomyosin 1 (tpm1) and poly(rC) binding protein 2 (pcbp2) are direct miR-21 targets. Taken together, our results not only reveal a large number of novel miR-21 regulated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of miR-21 regulation of zebrafish cardiac valvulogenesis and embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping