PUBLICATION
Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish
- Authors
- Dai, X., Shu, Y., Lou, Q., Tian, Q., Zhai, G., Song, J., Lu, S., Yu, H., He, J., Yin, Z.
- ID
- ZDB-PUB-170608-4
- Date
- 2017
- Source
- International Journal of Molecular Sciences 18(6): (Journal)
- Registered Authors
- He, Jiangyan, Yin, Zhan, Zhai, Gang
- Keywords
- differentiation, germ cell, knockout, maintenance, tdrd12, zebrafish
- MeSH Terms
-
- Phylogeny
- Animals
- Genotype
- Mutation
- Germ Cells/cytology*
- PubMed
- 28590408 Full text @ Int. J. Mol. Sci.
Abstract
The regularity of Piwi-interacting RNA (piRNA) biogenesis is crucial to germline development. Functioning as Piwi-interacting proteins, Tudor domain-related proteins (Tdrds) have been demonstrated to be involved in spermatogenesis and the piRNA pathway. In this study, zebrafish tdrd12 was identified, and the maternal and germ cell-specific expression patterns of zebrafish tdrd12 were observed. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent tdrd12 mutant zebrafish lines were generated. Although no defects were found during the generation of the primordial germ cells (PGCs) in the tdrd12-null fish progenies obtained from the heterozygous tdrd12 mutant parents, all Tdrd12-deficient fish developed into infertile males. The reduced numbers and eventually loss of the germ cells by 35 days post fertilization (dpf) led to masculinization and infertility of the Tdrd12-deficient fish. Meiosis defects of the germ cells in the tdrd12 mutants during the gonad-transitioning period were observed, revealing the indispensable functions of Tdrd12 in gametogenesis. Our studies demonstrated that zebrafish Tdrd12 is essential for germ cell development and maintenance.
Genes / Markers
Figure Gallery (7 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping