|ZFIN ID: ZDB-PUB-170402-5|
Inducible Inhibition of Gene Function with Photomorpholinos
|Source:||Methods in molecular biology (Clifton, N.J.) 1565: 51-57 (Chapter)|
|Registered Authors:||Sumanas, Saulius|
|Keywords:||Morpholinos Caged, Photoactivatable, Zebrafish|
|PubMed:||28364233 Full text @ Meth. Mol. Biol.|
Sumanas, S. (2017) Inducible Inhibition of Gene Function with Photomorpholinos. Methods in molecular biology (Clifton, N.J.). 1565:51-57.
ABSTRACTPhotoactivatable morpholinos (MO) allow specific temporal and spatial inhibition of gene function, which is not possible with conventional morpholino or genetic global gene knock-out approaches. Here, we describe an application of commercially available photoactivatable MO technology for specific gene inhibition in a zebrafish embryonic model and discuss the required controls related to the specificity and efficacy of this method. A similar approach should be also applicable to other model organisms.
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