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ZFIN ID: ZDB-PUB-170402-5
Inducible Inhibition of Gene Function with Photomorpholinos
Sumanas, S.
Date: 2017
Source: Methods in molecular biology (Clifton, N.J.)   1565: 51-57 (Chapter)
Registered Authors: Sumanas, Saulius
Keywords: Morpholinos Caged, Photoactivatable, Zebrafish
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian/metabolism
  • Gene Expression
  • Gene Knockdown Techniques
  • Gene Silencing*
  • Genes, Reporter
  • Morpholinos/chemistry
  • Morpholinos/genetics*
  • Oligonucleotides, Antisense/chemistry
  • Oligonucleotides, Antisense/genetics
  • Phenotype
  • Ultraviolet Rays
  • Zebrafish
  • Zebrafish Proteins/genetics
PubMed: 28364233 Full text @ Meth. Mol. Biol.
ABSTRACT
Photoactivatable morpholinos (MO) allow specific temporal and spatial inhibition of gene function, which is not possible with conventional morpholino or genetic global gene knock-out approaches. Here, we describe an application of commercially available photoactivatable MO technology for specific gene inhibition in a zebrafish embryonic model and discuss the required controls related to the specificity and efficacy of this method. A similar approach should be also applicable to other model organisms.
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