PUBLICATION
itFISH: Enhanced Staining by Iterative Fluorescent In Situ Hybridization
- Authors
- Row, R.H., Martin, B.L.
- ID
- ZDB-PUB-170321-5
- Date
- 2017
- Source
- Zebrafish 14(6): 578-580 (Journal)
- Registered Authors
- Martin, Benjamin, Row, Richard H.
- Keywords
- fluorescent in situ hybridization, iterative, neuromesodermal progenitor
- MeSH Terms
-
- Animals
- Fluorescent Dyes/analysis*
- In Situ Hybridization, Fluorescence/methods*
- Staining and Labeling/methods*
- Zebrafish/physiology*
- PubMed
- 28318417 Full text @ Zebrafish
Citation
Row, R.H., Martin, B.L. (2017) itFISH: Enhanced Staining by Iterative Fluorescent In Situ Hybridization. Zebrafish. 14(6):578-580.
Abstract
Fluorescent in situ hybridization (FISH) is an important tool for zebrafish research, particularly when observing the expression of two different genes in the same embryo. Peroxidase-catalyzed deposition of tyramide-conjugated dyes is a widely used and cost-effective approach to performing FISH. A major limitation of the technique is that it does not work well for weakly expressed genes. Here we present a method adapted from planarian research for use in zebrafish that provides a dramatic enhancement of weak staining. By iterating the antibody staining and development steps, a strong signal can be obtained from probes that were previously too weak to detect.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping