PUBLICATION

Green-Light-Induced Inactivation of Receptor Signaling Using Cobalamin-Binding Domains

Authors
Kainrath, S., Stadler, M., Reichhart, E., Distel, M., Janovjak, H.
ID
ZDB-PUB-170321-2
Date
2017
Source
Angewandte Chemie (International ed. in English)   56(16): 4608-4611 (Journal)
Registered Authors
Distel, Martin, Stadler, Manuela
Keywords
cobalamins, optogenetics, photochromism, receptors
MeSH Terms
none
PubMed
28319307 Full text @ Angew. Chem. Int. Ed. Engl.
Abstract
Optogenetics and photopharmacology provide spatiotemporally precise control over protein interactions and protein function in cells and animals. Optogenetic methods that are sensitive to green light and can be used to break protein complexes are not broadly available but would enable multichromatic experiments with previously inaccessible biological targets. Herein, we repurposed cobalamin (vitamin B12) binding domains of bacterial CarH transcription factors for green-light-induced receptor dissociation. In cultured cells, we observed oligomerization-induced cell signaling for the fibroblast growth factor receptor 1 fused to cobalamin-binding domains in the dark that was rapidly eliminated upon illumination. In zebrafish embryos expressing fusion receptors, green light endowed control over aberrant fibroblast growth factor signaling during development. Green-light-induced domain dissociation and light-inactivated receptors will critically expand the optogenetic toolbox for control of biological processes.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping