PUBLICATION

Identification of accessory olfactory system and medial amygdala in the zebrafish

Authors

Biechl, D., Tietje, K., Ryu, S., Grothe, B., Gerlach, G., Wullimann, M.F.

ID

ZDB-PUB-170316-8

Date

2017

Source

Scientific Reports 7: 44295 (Journal)

Registered Authors

Gerlach, Gabriele, Ryu, Soojin, Wullimann, Mario F.

Keywords

Differentiation, Olfactory bulb

MeSH Terms

Transcription Factors/genetics
Transcription Factors/metabolism
Carbocyanines/chemistry
Larva/anatomy & histology
Larva/cytology
Larva/physiology*
Fluorescent Dyes/chemistry
Olfactory Pathways/anatomy & histology
Olfactory Pathways/cytology
Olfactory Pathways/physiology*
Phosphorylation
Mitogen-Activated Protein Kinase 3/genetics
Mitogen-Activated Protein Kinase 3/metabolism
Odorants/analysis
Smell/physiology
Amygdala/anatomy & histology
Amygdala/cytology
Amygdala/physiology*
Animals
Gene Expression
Microscopy, Confocal
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Zebrafish/anatomy & histology
Zebrafish/physiology*
Imprinting, Psychological
Olfactory Receptor Neurons/physiology*
Olfactory Receptor Neurons/ultrastructure
Olfactory Bulb/anatomy & histology
Olfactory Bulb/cytology
Olfactory Bulb/physiology*

PubMed

28290515 Full text @ Sci. Rep.

Abstract

Zebrafish larvae imprint on visual and olfactory cues of their kin on day 5 and 6 postfertilization, respectively. Only imprinted (but not non-imprinted) larvae show strongly activated crypt (and some microvillous) cells demonstrated by pERK levels after subsequent exposure to kin odor. Here, we investigate the olfactory bulb of zebrafish larvae for activated neurons located at the sole glomerulus mdG2 which receives crypt cell input. Imprinted larvae show a significantly increased activation of olfactory bulb cells compared to non-imprinted larvae after exposure to kin odor. Surprisingly, pERK activated Orthopedia-positive cell numbers in the intermediate ventral telencephalic nucleus were higher in non-imprinted, kin odor stimulated larvae compared to control and to kin-odor stimulated imprinted larvae and control. Moreover, DiI tracing experiments in adult zebrafish show a neuronal circuit from crypt/microvillous olfactory sensory neurons via dorsomedial olfactory bulb and intermediate ventral telencephalic nucleus (thus, arguably the teleostean medial amygdala) to tuberal hypothalamus, demonstrating for the first time an accessory olfactory system in teleosts.

Genes / Markers

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