PUBLICATION
Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors
- Authors
- Marques, C.L., Cancela, M.L., Laizé, V.
- ID
- ZDB-PUB-170214-98
- Date
- 2016
- Source
- Gene 576: 229-36 (Journal)
- Registered Authors
- Cancela, Leonor
- Keywords
- Myocyte enhancer factor 2C (MEF2C), Promoter activity, Runt-related transcription factor 3 (RUNX3), SRY (sex determining region Y)-box 9b (SOX9b), Transcriptional regulation, v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS1)
- MeSH Terms
-
- Animals
- Binding Sites
- Bone Morphogenetic Protein 2/genetics*
- Bone Morphogenetic Protein 2/metabolism
- Bone and Bones/physiology
- Cartilage/physiology
- Core Binding Factor Alpha 3 Subunit/genetics
- Core Binding Factor Alpha 3 Subunit/metabolism
- Core Binding Factor beta Subunit/genetics
- Core Binding Factor beta Subunit/metabolism
- Evolution, Molecular
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Gene Expression Regulation*
- HEK293 Cells
- Humans
- MEF2 Transcription Factors/genetics
- Promoter Regions, Genetic
- Proto-Oncogene Protein c-ets-1/genetics
- SOX9 Transcription Factor/genetics
- SOX9 Transcription Factor/metabolism
- Sea Bream/genetics*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish Proteins/genetics
- PubMed
- 26456102 Full text @ Gene
Citation
Marques, C.L., Cancela, M.L., Laizé, V. (2016) Transcriptional regulation of gilthead seabream bone morphogenetic protein (BMP) 2 gene by bone- and cartilage-related transcription factors. Gene. 576:229-36.
Abstract
Bone morphogenetic protein (BMP) 2 belongs to the transforming growth factor β (TGFβ) superfamily of cytokines and growth factors. While it plays important roles in embryo morphogenesis and organogenesis, BMP2 is also critical to bone and cartilage formation. Protein structure and function have been remarkably conserved throughout evolution and BMP2 transcription has been proposed to be tightly regulated, although few data is available. In this work we report the cloning and functional analysis of gilthead seabream BMP2 promoter. As in other vertebrates, seabream BMP2 gene has a 5′ non-coding exon, a feature already present in DPP gene, the fruit fly ortholog of vertebrate BMP2 gene, and maintained throughout evolution. In silico analysis of seabream BMP2 promoter revealed several binding sites for bone and cartilage related transcription factors (TFs) and their functionality was evaluated using promoter-luciferase constructions and TF-expressing vectors. Runt-related transcription factor 3 (RUNX3) was shown to negatively regulate BMP2 transcription and combination with the core binding factor β (CBFβ) further reduced transcriptional activity of the promoter. Although to a lesser extent, myocyte enhancer factor 2C (MEF2C) had also a negative effect on the regulation of BMP2 gene transcription, when associated with SRY (sex determining region Y)-box 9 (SOX9b). Finally, v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS1) was able to slightly enhance BMP2 transcription. Data reported here provides new insights toward the better understanding of the transcriptional regulation of BMP2 gene in a bone and cartilage context.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping