PUBLICATION
The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo
- Authors
- Carvajal-Gonzalez, J.M., Balmer, S., Mendoza, M., Dussert, A., Collu, G., Roman, A.C., Weber, U., Ciruna, B., Mlodzik, M.
- ID
- ZDB-PUB-170214-200
- Date
- 2015
- Source
- Nature communications 6: 6751 (Journal)
- Registered Authors
- Ciruna, Brian
- Keywords
- Cell polarity, Membrane trafficking
- MeSH Terms
-
- Adaptor Protein Complex 1/genetics*
- Early Growth Response Protein 2/genetics
- Early Growth Response Protein 2/metabolism
- Drosophila/embryology*
- Drosophila/metabolism
- Carrier Proteins/metabolism
- Dishevelled Proteins
- ADP-Ribosylation Factor 1/genetics*
- Animals, Genetically Modified
- Membrane Proteins/metabolism
- LIM Domain Proteins/metabolism
- Wings, Animal/embryology*
- Gene Expression Regulation, Developmental
- Drosophila Proteins/genetics*
- Drosophila Proteins/metabolism
- Cadherins/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Adaptor Proteins, Signal Transducing/metabolism
- Cell Polarity/genetics*
- MyoD Protein/genetics
- MyoD Protein/metabolism
- Frizzled Receptors/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism
- DNA-Binding Proteins/metabolism
- Animals
- Phosphoproteins/metabolism
- PubMed
- 25849195 Full text @ Nat. Commun.
Citation
Carvajal-Gonzalez, J.M., Balmer, S., Mendoza, M., Dussert, A., Collu, G., Roman, A.C., Weber, U., Ciruna, B., Mlodzik, M. (2015) The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo. Nature communications. 6:6751.
Abstract
A key step in generating planar cell polarity (PCP) is the formation of restricted junctional domains containing Frizzled/Dishevelled/Diego (Fz/Dsh/Dgo) or Van Gogh/Prickle (Vang/Pk) complexes within the same cell, stabilized via Flamingo (Fmi) across cell membranes. Although models have been proposed for how these complexes acquire and maintain their polarized localization, the machinery involved in moving core PCP proteins around cells remains unknown. We describe the AP-1 adaptor complex and Arf1 as major regulators of PCP protein trafficking in vivo. AP-1 and Arf1 disruption affects the accumulation of Fz/Fmi and Vang/Fmi complexes in the proximo-distal axis, producing severe PCP phenotypes. Using novel tools, we demonstrate a direct and specific Arf1 involvement in Fz trafficking in vivo. Moreover, we uncover a conserved Arf1 PCP function in vertebrates. Our data support a model whereby the trafficking machinery plays an important part during PCP establishment, promoting formation of polarized PCP-core complexes in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping