ZFIN ID: ZDB-PERS-020116-4
Ciruna, Brian
Email: ciruna@sickkids.ca
URL:
Affiliation: Ciruna Lab
Address: Program in Developmental & Stem Cell Biology The Hospital for Sick Children 101 College Street MaRS East Tower, Room 11-306 Toronto, Ontario, M5G 1L7 Canada
Country: Canada
Phone: (416) 813-7654 x2050
Fax: (416) 813-5252
ORCID ID:


BIOGRAPHY AND RESEARCH INTERESTS
RESEARCH INTERESTS

-Molecular genetic regulation of embryonic development
-Cell polarity and morphogenesis
-Neurulation and neural tube closure defects
-Zebrafish models of development and disease

RESEARCH ACTIVITIES

We are interested in understanding the molecular and genetic mechanisms that regulate early development, with specific emphasis on the planar cell polarity (PCP) signalling pathway and its role in embryonic morphogenesis.

Correct polarity is essential for normal cellular function. The characteristic apical-basolateral polarity of epithelial cells, for example, is required for directional ion transport and deposition of the basal lamina. In addition, polarity may also exist across the plane of an epithelium or field of cells. In vertebrates, this planar cell polarity (or PCP) instructs polarized cell rearrangements and morphogenetic processes that structure and shape the developing embryo. At neurulation, defects in PCP signalling are thought to cause spina bifida and anencephaly - neural tube closure defects that affect one in every 1000 human births. PCP signalling has also been implicated in skin development, polarization of the inner ear sensory epithelium, cardiac development and cancer. However, little is known about how PCP signals ultimately regulate these important cellular and physiological processes.

We are using the zebrafish as a model organism to investigate the fundamental mechanisms by which cell polarity is established, maintained and interpreted in the course of vertebrate embryonic development. By combining powerful live microscopic imaging capabilities with the genetic and embryological techniques afforded by zebrafish research, we are examining the dynamic regulation of PCP signalling at a sub-cellular level. Furthermore, we are employing forward genetic and candidate gene approaches to screen for novel regulators and modifiers of PCP signalling. We hope to gain insight into how regulation of cell polarity functions in normal development, and how aberrations in PCP signalling contribute to congenital malformations and disease.


PUBLICATIONS
Van Gennip, J.L.M., Boswell, C.W., Ciruna, B. (2018) Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis. Science advances. 4:eaav1781
Konjikusic, M.J., Yeetong, P., Boswell, C.W., Lee, C., Roberson, E.C., Ittiwut, R., Suphapeetiporn, K., Ciruna, B., Gurnett, C.A., Wallingford, J.B., Shotelersuk, V., Gray, R.S. (2018) Mutations in Kinesin family member 6 reveal specific role in ependymal cell ciliogenesis and human neurological development. PLoS Genetics. 14:e1007817
Jussila, M., Ciruna, B. (2017) Zebrafish models of non-canonical Wnt/planar cell polarity signalling: fishing for valuable insight into vertebrate polarized cell behavior. Wiley interdisciplinary reviews. Developmental biology. 6(3)
Boswell, C.W., Ciruna, B. (2017) Understanding Idiopathic Scoliosis: A New Zebrafish School of Thought. Trends in genetics : TIG. 33(3):183-196
Hanwell, D., Hutchinson, S.A., Collymore, C., Bruce, A.E., Louis, R., Ghalami, A., Allison, W.T., Ekker, M., Eames, B.F., Childs, S., Kurrasch, D.M., Gerlai, R., Thiele, T., Scott, I., Ciruna, B., Dowling, J.J., McFarlane, S., Huang, P., Wen, X.Y., Akimenko, M.A., Waskiewicz, A.J., Drapeau, P., Babiuk, L.A., Dragon, D., Smida, A., Buret, A.G., O'Grady, E., Wilson, J., Sowden-Plunkett, L., Tropepe, V. (2016) Restrictions on the Importation of Zebrafish into Canada Associated with Spring Viremia of Carp Virus. Zebrafish. 13 Suppl 1:S153-63
Grimes, D.T., Boswell, C.W., Morante, N.F., Henkelman, R.M., Burdine, R.D., Ciruna, B. (2016) Zebrafish models of idiopathic scoliosis link cerebrospinal fluid flow defects to spine curvature. Science (New York, N.Y.). 352:1341-4
Carvajal-Gonzalez, J.M., Balmer, S., Mendoza, M., Dussert, A., Collu, G., Roman, A.C., Weber, U., Ciruna, B., Mlodzik, M. (2015) The clathrin adaptor AP-1 complex and Arf1 regulate planar cell polarity in vivo. Nature communications. 6:6751
Hayes, M., Gao, X., Yu, L.X., Paria, N., Henkelman, R.M., Wise, C.A., Ciruna, B. (2014) ptk7 mutant zebrafish models of congenital and idiopathic scoliosis implicate dysregulated Wnt signalling in disease. Nature communications. 5:4777
Superina, S., Borovina, A., and Ciruna, B. (2014) Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology. 387(2):154-166
Borovina, A., and Ciruna, B. (2013) IFT88 Plays a Cilia- and PCP-Independent Role in Controlling Oriented Cell Divisions during Vertebrate Embryonic Development. Cell Reports. 5(1):37-43
Pan, Y.A., Freundlich, T., Weissman, T.A., Schoppik, D., Wang, X.C., Zimmerman, S., Ciruna, B., Sanes, J.R., Lichtman, J.W., and Schier, A.F. (2013) Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish. Development (Cambridge, England). 140(13):2835
Hayes, M., Naito, M., Daulat, A., Angers, S., and Ciruna, B. (2013) Ptk7 promotes non-canonical Wnt/PCP-mediated morphogenesis and inhibits Wnt/beta-catenin-dependent cell fate decisions during vertebrate development. Development (Cambridge, England). 140(8):1807-1818
Schoenebeck, J.J., Hutchinson, S.A., Byers, A., Beale, H.C., Carrington, B., Faden, D.L., Rimbault, M., Decker, B., Kidd, J.M., Sood, R., Boyko, A.R., Fondon, J.W., Wayne, R.K., Bustamante, C.D., Ciruna, B., and Ostrander, E.A. (2012) Variation of BMP3 Contributes to Dog Breed Skull Diversity. PLoS Genetics. 8(8):e1002849
Persaud, A., Alberts, P., Hayes, M., Guettler, S., Clarke, I., Sicheri, F., Dirks, P., Ciruna, B., and Rotin, D. (2011) Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function. The EMBO journal. 30(16):3259-73
Borovina, A., Superina, S., Voskas, D., and Ciruna, B. (2010) Vangl2 directs the posterior tilting and asymmetric localization of motile primary cilia. Nature cell biology. 12(4):407-412
Calarco, J.A., Superina, S., O'Hanlon, D., Gabut, M., Raj, B., Pan, Q., Skalska, U., Clarke, L., Gelinas, D., van der Kooy, D., Zhen, M., Ciruna, B., and Blencowe, B.J. (2009) Regulation of vertebrate nervous system alternative splicing and development by an SR-related protein. Cell. 138(5):898-910
Yin, C., Ciruna, B., and Solnica-Krezel, L. (2009) Convergence and extension movements during vertebrate gastrulation. Current topics in developmental biology. 89:163-192
Bennett, J.T., Stickney, H.L., Choi, W.Y., Ciruna, B., Talbot, W.S., and Schier, A.F. (2007) Maternal nodal and zebrafish embryogenesis. Nature. 450(7167):E1-E4
Wang, W., Liu, X., Gelinas, D., Ciruna, B., and Sun, Y. (2007) A fully automated robotic system for microinjection of zebrafish embryos. PLoS One. 2(9):e862
Ciruna, B., Jenny, A., Lee, D., Mlodzik, M., and Schier, A.F. (2006) Planar cell polarity signalling couples cell division and morphogenesis during neurulation. Nature. 439(7073):220-224
Ciruna, B., Weidinger, G., Knaut, H., Thisse, B., Thisse, C., Raz, E., and Schier, A.F. (2002) Production of maternal-zygotic mutant zebrafish by germ-line replacement. Proceedings of the National Academy of Sciences of the United States of America. 99(23):14919-14924

NON-ZEBRAFISH PUBLICATIONS
Zhao H, Yang Y, Partanen J, Ciruna BG, Rossant J, Robinson ML (2006) Fibroblast growth factor receptor 1 (Fgfr1) is not essential for lens fiber differentiation in mice. Molecular Vision 12: 15-25.

Ciruna B, Rossant J (2001) FGF signaling regulates mesoderm cell fate specification and morphogenetic movement at the primitive streak. Developmental Cell 1(1): 37-49.

Saxton TM, Ciruna BG, Holmyard D, Kulkarni S, Harpal K, Rossant J, Pawson T (2000) The SH2 tyrosine phosphatase shp2 is required for mammalian limb development. Nature Genetics 24(4): 420-3.

Ciruna BG, Rossant J (1999) Expression of the T-box gene Eomesodermin during early mouse development. Mechanisms of Development 81(1-2): 199-203.

Tropepe V, Sibilia M, Ciruna BG, Rossant J, Wagner EF, van der Kooy D (1999) Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Developmental Biology 208(1): 166-88.

Ciruna BG, Schwartz L, Harpal K, Yamaguchi TP, Rossant J (1997) Chimeric analysis of fibroblast growth factor receptor-1 (Fgfr1) function: a role for FGFR1 in morphogenetic movement through the primitive streak. Development 124(14): 2829-41.

Rossant J, Ciruna B, Partanen J (1997) FGF signaling in mouse gastrulation and anteroposterior patterning. Cold Spring Harb Symp Quant Biol. 62: 127-33.