PUBLICATION
Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin
- Authors
- Cardeira, J., Gavaia, P.J., Fernández, I., Cengiz, I.F., Moreira-Silva, J., Oliveira, J.M., Reis, R.L., Cancela, M.L., Laizé, V.
- ID
- ZDB-PUB-161220-4
- Date
- 2016
- Source
- Scientific Reports 6: 39191 (Journal)
- Registered Authors
- Cancela, Leonor
- Keywords
- Musculoskeletal models, Zebrafish
- MeSH Terms
-
- Animal Fins/pathology
- Animal Fins/physiology*
- Animals
- Bone Regeneration/drug effects
- Bone Regeneration/physiology
- Bone and Bones/physiology
- Calcification, Physiologic/drug effects
- Regeneration/drug effects
- Regeneration/physiology*
- Tretinoin/pharmacology
- Warfarin/pharmacology
- X-Ray Microtomography
- Zebrafish/physiology*
- PubMed
- 27991522 Full text @ Sci. Rep.
Citation
Cardeira, J., Gavaia, P.J., Fernández, I., Cengiz, I.F., Moreira-Silva, J., Oliveira, J.M., Reis, R.L., Cancela, M.L., Laizé, V. (2016) Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin. Scientific Reports. 6:39191.
Abstract
The ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through methods that require optimization and standardization. Here, we present an improved methodology to characterize and analyse overall caudal fin and bone regeneration in adult zebrafish. First, regenerated and mineralized areas are evaluated through broad, rapid and specific chronological and morphometric analysis in alizarin red stained fins. Then, following a more refined strategy, the intensity of the staining within a 2D longitudinal plane is determined through pixel intensity analysis, as an indicator of density or thickness/volume. The applicability of this methodology on live specimens, to reduce animal experimentation and provide a tool for in vivo tracking of the regenerative process, was successfully demonstrated. Finally, the methodology was validated on retinoic acid- and warfarin-treated specimens, and further confirmed by micro-computed tomography. Because it is easily implementable, accurate and does not require sophisticated equipment, the present methodology will certainly provide valuable technical standardization for research in tissue engineering, regenerative medicine and skeletal biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping