ZFIN ID: ZDB-PUB-161204-9
Comparative study of dual-pulsed 1,064 nm Q-switched Nd:YAG laser and single-pulsed 1,064 nm Q-switched Nd:YAG laser by using zebrafish model and prospective split-face analysis of facial melasma
Jang, H.W., Chun, S.H., Park, H.C., Ryu, H.J., Kim, I.H.
Date: 2017
Source: Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology   19(2): 114-123 (Journal)
Registered Authors: Park, Hae-Chul
Keywords: Melasma, Nd:YAG, Zebrafish
MeSH Terms:
  • Adult
  • Aluminum
  • Animals
  • Asian Continental Ancestry Group
  • Cell Death
  • Face/radiation effects*
  • Female
  • Humans
  • Lasers, Solid-State/therapeutic use*
  • Low-Level Light Therapy/methods*
  • Melanosis/radiotherapy*
  • Middle Aged
  • Neodymium
  • Patient Satisfaction
  • Prospective Studies
  • Yttrium
  • Zebrafish
PubMed: 27911108 Full text @ J. Cosmet. Laser Ther.
Recently dual-pulsed low-fluence 1064-nm Q-switched Nd:YAG (QSNY) laser has been developed for reducing complication during melasma treatment.
Comparison of the efficacy and safety between dual-pulsed mode and single-pulsed mode for the treatment of melasma.
In preclinical study, adult zebrafish were irradiated with dual-pulsed and single-pulsed mode. Changes of melanophore and cell death were assessed. In split-face clinical study, dual-pulsed and single-pulsed mode were irradiated on the left and right side of the face, respectively. L* value, clinical digital photos, modified Melasma Area and Severity Index (MASI) scores, and side effects were measured.
As compared to single-pulsed mode and dual-pulsed mode with longer intervals, zebrafish melanophore was cleared quickly at dual-pulsed mode with 80-μsec interval and 0.3 J/cm2 fluence. Dual-pulsed mode showed the least regeneration of melanophore at 4 weeks after irradiation and no cell death was observed with 80-μsec interval. Both pulse modes improved melasma significantly but modified MASI score and L* value were not significantly different between each other. Lesser pain and shorter duration of post-laser erythema were observed with dual-pulsed mode.
Dual-pulsed mode was as effective as single-pulsed mode for the treatment of melasma and revealed less side effects.