ZFIN ID: ZDB-PUB-161129-4
Internal epitope tagging informed by relative lack of sequence conservation
Burg, L., Zhang, K., Bonawitz, T., Grajevskaja, V., Bellipanni, G., Waring, R., Balciunas, D.
Date: 2016
Source: Scientific Reports   6: 36986 (Journal)
Registered Authors: Balciunas, Darius, Bellipanni, Gianfranco
Keywords: CRISPR-Cas9 genome editing, Genetic engineering, Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Antibodies/genetics
  • Conserved Sequence/genetics*
  • Epitopes/genetics*
  • Proteins/genetics
  • Sequence Alignment/methods
  • Zebrafish
PubMed: 27892520 Full text @ Sci. Rep.
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.