PUBLICATION

Radial glia regulate vascular patterning around the developing spinal cord

Authors
Matsuoka, R.L., Marass, M., Avdesh, A., Helker, C.S., Maischein, H.M., Grosse, A.S., Kaur, H., Lawson, N.D., Herzog, W., Stainier, D.Y.
ID
ZDB-PUB-161118-6
Date
2016
Source
eLIFE   5: (Journal)
Registered Authors
Helker, Christian, Herzog, Wiebke, Lawson, Nathan, Maischein, Hans-Martin, Matsuoka, Ryota, Stainier, Didier
Keywords
developmental biology, neuroscience, stem cells, zebrafish
MeSH Terms
  • Zebrafish Proteins/genetics*
  • Vascular Endothelial Growth Factor Receptor-1/genetics*
  • Neuroglia/metabolism
  • Endothelial Cells/metabolism
  • Neural Stem Cells/metabolism
  • Vascular Endothelial Growth Factor A/genetics*
  • Cell Differentiation/genetics*
  • Gene Expression Regulation
  • Mosaicism
  • Spinal Cord/blood supply
  • Spinal Cord/growth & development
  • Spinal Cord/metabolism*
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Organogenesis/genetics*
  • Animals
  • Signal Transduction/genetics
  • Blood Vessels/growth & development
  • Blood Vessels/metabolism
(all 19)
PubMed
27852438 Full text @ Elife
Abstract
Vascular networks surrounding individual organs are important for their development, maintenance, and function; however, how these networks are assembled remains poorly understood. Here we show that CNS progenitors, referred to as radial glia, modulate vascular patterning around the spinal cord by acting as negative regulators. We found that radial glia ablation in zebrafish embryos leads to excessive sprouting of the trunk vessels around the spinal cord, and exclusively those of venous identity. Mechanistically, we determined that radial glia control this process via the Vegf decoy receptor sFlt1: sflt1 mutants exhibit the venous over-sprouting observed in radial glia-ablated larvae, and sFlt1 overexpression rescues it. Genetic mosaic analyses show that sFlt1 function in trunk endothelial cells can limit their over-sprouting. Together, our findings identify CNS-resident progenitors as critical angiogenic regulators that determine the precise patterning of the vasculature around the spinal cord, providing novel insights into vascular network formation around developing organs.
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Marker Marker Type Name
CeruleanEFGCerulean
EGFPEFGEGFP
GAL4EFGGAL4
GAL4FFEFGGAL4FF
GFPEFGGFP
mCherryEFGmCherry
NTREFGNTR
RFPEFGRFP
TagBFPEFGTagBFP
YFPEFGYFP
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Citation
Matsuoka, R.L., Marass, M., Avdesh, A., Helker, C.S., Maischein, H.M., Grosse, A.S., Kaur, H., Lawson, N.D., Herzog, W., Stainier, D.Y. (2016) Radial glia regulate vascular patterning around the developing spinal cord. eLIFE. 5.