PUBLICATION
Radial glia regulate vascular patterning around the developing spinal cord
- Authors
- Matsuoka, R.L., Marass, M., Avdesh, A., Helker, C.S., Maischein, H.M., Grosse, A.S., Kaur, H., Lawson, N.D., Herzog, W., Stainier, D.Y.
- ID
- ZDB-PUB-161118-6
- Date
- 2016
- Source
- eLIFE 5: (Journal)
- Registered Authors
- Helker, Christian, Herzog, Wiebke, Lawson, Nathan, Maischein, Hans-Martin, Matsuoka, Ryota, Stainier, Didier
- Keywords
- developmental biology, neuroscience, stem cells, zebrafish
- MeSH Terms
-
- Vascular Endothelial Growth Factor Receptor-1/genetics*
- Signal Transduction/genetics
- Animals
- Neural Stem Cells/metabolism
- Cell Differentiation/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish/genetics
- Zebrafish/growth & development
- Gene Expression Regulation
- Neuroglia/metabolism
- Endothelial Cells/metabolism
- Organogenesis/genetics*
- Blood Vessels/growth & development
- Blood Vessels/metabolism
- Vascular Endothelial Growth Factor A/genetics*
- Spinal Cord/blood supply
- Spinal Cord/growth & development
- Spinal Cord/metabolism*
- Mosaicism
- PubMed
- 27852438 Full text @ Elife
Citation
Matsuoka, R.L., Marass, M., Avdesh, A., Helker, C.S., Maischein, H.M., Grosse, A.S., Kaur, H., Lawson, N.D., Herzog, W., Stainier, D.Y. (2016) Radial glia regulate vascular patterning around the developing spinal cord. eLIFE. 5.
Abstract
Vascular networks surrounding individual organs are important for their development, maintenance, and function; however, how these networks are assembled remains poorly understood. Here we show that CNS progenitors, referred to as radial glia, modulate vascular patterning around the spinal cord by acting as negative regulators. We found that radial glia ablation in zebrafish embryos leads to excessive sprouting of the trunk vessels around the spinal cord, and exclusively those of venous identity. Mechanistically, we determined that radial glia control this process via the Vegf decoy receptor sFlt1: sflt1 mutants exhibit the venous over-sprouting observed in radial glia-ablated larvae, and sFlt1 overexpression rescues it. Genetic mosaic analyses show that sFlt1 function in trunk endothelial cells can limit their over-sprouting. Together, our findings identify CNS-resident progenitors as critical angiogenic regulators that determine the precise patterning of the vasculature around the spinal cord, providing novel insights into vascular network formation around developing organs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping