PUBLICATION

MicroRNA-126a Directs Lymphangiogenesis Through Interacting With Chemokine and Flt4 Signaling in Zebrafish

Authors
Chen, J., Zhu, R.F., Li, F.F., Liang, Y.L., Wang, C., Qin, Y.W., Huang, S., Zhao, X.X., Jing, Q.
ID
ZDB-PUB-161030-17
Date
2016
Source
Arteriosclerosis, Thrombosis, and Vascular Biology   36(12): 2381-2393 (Journal)
Registered Authors
Jing, Qing, Liang, Yulai, Li, Fangfang, Rongfang, Zhu
Keywords
endothelial cells, lymphangiogenesis, microRNAs, thoracic duct, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • CRISPR-Cas Systems
  • Cell Movement
  • Cell Proliferation
  • Chemokine CXCL12/genetics
  • Chemokine CXCL12/metabolism*
  • Computational Biology
  • Gene Expression Regulation, Developmental
  • Gene Silencing
  • Genotype
  • Lymphangiogenesis*
  • Lymphography
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Microscopy, Confocal
  • Morpholinos/genetics
  • Morpholinos/metabolism
  • Phenotype
  • Signal Transduction*
  • Thoracic Duct/embryology
  • Thoracic Duct/metabolism*
  • Time Factors
  • Time-Lapse Imaging
  • Vascular Endothelial Growth Factor Receptor-3/genetics
  • Vascular Endothelial Growth Factor Receptor-3/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
27789478 Full text @ Arterio., Thromb., and Vas. Bio.
Abstract
MicroRNA-126 (miR-126) is an endothelium-enriched miRNA and functions in vascular integrity and angiogenesis. The application of miRNA as potential biomarker and therapy target has been widely investigated in various pathological processes. However, its role in lymphatic diseases had not been widely explored. We aimed to reveal the role of miR-126 in lymphangiogenesis and the regulatory signaling pathways for potential targets of therapy.
Loss-of-function studies using morpholino oligonucleotides and CRISPR/Cas9 system showed that silencing of miR-126a severely affected the formation of parachordal lymphangioblasts and thoracic duct in zebrafish embryos, although their development in miR-126b knockdown embryos was normal. Expression analyses by in situ hybridization and immunofluorescence indicated that miR-126a was expressed in lymphatic vessels, as well as in blood vessels. Time-lapse confocal imaging assay further revealed that knockdown of miR-126a blocked both lymphangiogenic sprouts budding from the posterior cardinal vein and lymphangioblasts extension along horizontal myoseptum. Bioinformatics analysis and in vivo report assay identified that miR-126a upregulated Cxcl12a by targeting its 5' untranslated region. Moreover, loss- and gain-of-function studies revealed that Cxcl12a signaling acted downstream of miR-126a during parachordal lymphangioblast extension, whereby Flt4 signaling acts as a cooperator of miR-126a, allowing it to modulate lymphangiogenic sprout formation.
These findings demonstrate that miR-126a directs lymphatic endothelial cell sprouting and extension by interacting with Cxcl12a-mediated chemokine signaling and Vegfc-Flt4 signal axis. Our results suggest that these key regulators of lymphangiogenesis may be involved in lymphatic pathogenesis of cardiovascular diseases.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping