Expression of miRNA-122 Induced by Liver Toxicants in Zebrafish
- Nam, H.S., Hwang, K.S., Jeong, Y.M., Ryu, J.I., Choi, T.Y., Bae, M.A., Son, W.C., You, K.H., Son, H.Y., Kim, C.H.
- BioMed Research International 2016: 1473578 (Journal)
- Registered Authors
- Choi, Tae-Young, Kim, Cheol-Hee
- MeSH Terms
- Biological Assay/methods*
- Chemical and Drug Induced Liver Injury/genetics*
- Drug Evaluation, Preclinical/methods*
- Toxicity Tests/methods*
- 27563662 Full text @ Biomed Res. Int.
Nam, H.S., Hwang, K.S., Jeong, Y.M., Ryu, J.I., Choi, T.Y., Bae, M.A., Son, W.C., You, K.H., Son, H.Y., Kim, C.H. (2016) Expression of miRNA-122 Induced by Liver Toxicants in Zebrafish. BioMed Research International. 2016:1473578.
MicroRNA-122 (miRNA-122), also known as liver-specific miRNA, has recently been shown to be a potent biomarker in response to liver injury in mammals. The objective of this study was to examine its expression in response to toxicant treatment and acute liver damage, using the zebrafish system as an alternative model organism. For the hepatotoxicity assay, larval zebrafish were arrayed in 24-well plates. Adult zebrafish were also tested and arrayed in 200 mL cages. Animals were exposed to liver toxicants (tamoxifen or acetaminophen) at various doses, and miRNA-122 expression levels were analyzed using qRT-PCR in dissected liver, brain, heart, and intestine, separately. Our results showed no significant changes in miRNA-122 expression level in tamoxifen-treated larvae; however, miRNA-122 expression was highly induced in tamoxifen-treated adults in a tissue-specific manner. In addition, we observed a histological change in adult liver (0.5 μM) and cell death in larval liver (5 μM) at different doses of tamoxifen. These results indicated that miRNA-122 may be utilized as a liver-specific biomarker for acute liver toxicity in zebrafish.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and NotesCorrection.