PUBLICATION
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
- Authors
- McKee, R.A., Wingert, R.A.
- ID
- ZDB-PUB-160809-3
- Date
- 2016
- Source
- Journal of visualized experiments : JoVE (113): (Journal)
- Registered Authors
- McKee, Robert, Wingert, Rebecca
- Keywords
- none
- MeSH Terms
-
- Acute Kidney Injury/chemically induced*
- Animals
- Disease Models, Animal*
- Embryo, Nonmammalian/drug effects
- Humans
- Kidney/physiopathology
- Microinjections*
- Zebrafish/embryology
- PubMed
- 27500823 Full text @ J. Vis. Exp.
Citation
McKee, R.A., Wingert, R.A. (2016) Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury. Journal of visualized experiments : JoVE. (113).
Abstract
The kidneys are susceptible to harm from exposure to chemicals they filter from the bloodstream. This can lead to organ injury associated with a rapid decline in renal function and development of the clinical syndrome known as acute kidney injury (AKI). Pharmacological agents used to treat medical circumstances ranging from bacterial infection to cancer, when administered individually or in combination with other drugs, can initiate AKI. Zebrafish are a useful animal model to study the chemical effects on renal function in vivo, as they form an embryonic kidney comprised of nephron functional units that are conserved with higher vertebrates, including humans. Further, zebrafish can be utilized to perform genetic and chemical screens, which provide opportunities to elucidate the cellular and molecular facets of AKI and develop therapeutic strategies such as the identification of nephroprotective molecules. Here, we demonstrate how microinjection into the zebrafish embryo can be utilized as a paradigm for nephrotoxin studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping