ZFIN ID: ZDB-PUB-160729-8
A Practical Guide to Light Sheet Microscopy
Bennett, D.V., Ahrens, M.B.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1451: 321-31 (Chapter)
Registered Authors: Ahrens, Misha, Bennett, Davis
Keywords: Calcium indicators, Imaging, Light sheet, Microscopy, Systems neuroscience, Zebrafish
MeSH Terms:
  • Animals
  • Brain
  • Larva
  • Microscopy/methods*
  • Microscopy, Fluorescence
  • Zebrafish
PubMed: 27464818 Full text @ Meth. Mol. Biol.
Citation
Bennett, D.V., Ahrens, M.B. (2016) A Practical Guide to Light Sheet Microscopy. Methods in molecular biology (Clifton, N.J.). 1451:321-31.
ABSTRACT
Light sheet fluorescence microscopy is an efficient method for imaging large volumes of biological tissue, including brains of larval zebrafish, at high spatial and fairly high temporal resolution with minimal phototoxicity.Here, we provide a practical guide for those who intend to build a light sheet microscope for fluorescence imaging in live larval zebrafish brains or other tissues.
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