Bennett, D.V., Ahrens, M.B. (2016) A Practical Guide to Light Sheet Microscopy. Methods in molecular biology (Clifton, N.J.). 1451:321-31.
Light sheet fluorescence microscopy is an efficient method for imaging large volumes of biological tissue, including brains of larval zebrafish, at high spatial and fairly high temporal resolution with minimal phototoxicity.Here, we provide a practical guide for those who intend to build a light sheet microscope for fluorescence imaging in live larval zebrafish brains or other tissues.