PUBLICATION

Identification of apoptosis and macrophage migration events in paraquat-induced oxidative stress using a zebrafish model

Authors
Wang, Q., Liu, S., Hu, D., Wang, Z., Wang, L., Wu, T., Wu, Z., Mohan, C., Peng, A.
ID
ZDB-PUB-160612-3
Date
2016
Source
Life sciences   157: 116-24 (Journal)
Registered Authors
Wang, Qiang
Keywords
Apoptosis, Macrophage migration, Oxidative stress, Paraquat, Zebrafish model
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Apoptosis/drug effects*
  • Cell Movement/drug effects*
  • Herbicides/toxicity*
  • Macrophages/drug effects*
  • Models, Animal*
  • Oxidative Stress/drug effects*
  • Paraquat/toxicity*
  • Zebrafish
PubMed
27288846 Full text @ Life Sci.
Abstract
Paraquat (PQ) is a pesticide highly toxic to human beings, and a well-known trigger of oxidative stress. Although several animal models of PQ poisoning have been developed, some disadvantages limit their application in vivo. A zebrafish model was used in the present study to better define mechanisms of oxidative stress injury induced by PQ.
The toxicity of PQ was evaluated in the AB strain of zebrafish, and apoptosis was assessed by acridine orange staining. Macrophage migration was identified using the TG (zlyz:EGFP) transgenic strain, and angiogenesis was observed using the fli1a-EGFP casper strain. Following the validation of gene changes by zebrafish-based in vivo quantitative real-time PCR, network analysis was performed using the Ingenuity Pathway Analysis software.
We first established the LC50 of PQ in the zebrafish model, and then found that robust oxidative stress and antioxidant genes were activated after PQ exposure. Moreover, apoptosis and distinct macrophage activation and migration were identified for the first time in PQ-exposed zebrafish. Utilizing this model, both extrinsic and intrinsic pathways involved in PQ-induced apoptosis were elucidated. We further demonstrated that macrophage migration was specifically induced by PQ, and that Rho family members and JNK-MMP13 signaling participated in this process.
Zebrafish is a promising tool for investigating the mechanisms of oxidative stress injury induced by PQ, and for screening effective anti-oxidant drugs.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping