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Research
General Information
ZIRC
ZFIN ID: ZDB-PERS-000914-13
Kawahara, Atsuo
Email: akawahara@yamanashi.ac.jp
URL:
Affiliation: Kawahara Lab
Address: Laboratory for Developmental Biology Center for Medical Education and Sciences Graduate School of Medical Science University of Yamanashi Shimokato 1110, Chuo, Yamanashi, 409-3898, Japan
Country: Japan
Phone:
Fax:
Orcid ID:


BIOGRAPHY AND RESEARCH INTERESTS


PUBLICATIONS
Iwasaki, R., Tsuge, K., Kishimoto, K., Hayashi, Y., Iwaana, T., Hohjoh, H., Inazumi, T., Kawahara, A., Tsuchiya, S., Sugimoto, Y. (2019) Essential role of prostaglandin E2 and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis. Scientific Reports. 9:7650
Hirayama, J., Alifu, Y., Hamabe, R., Yamaguchi, S., Tomita, J., Maruyama, Y., Asaoka, Y., Nakahama, K.I., Tamaru, T., Takamatsu, K., Takamatsu, N., Hattori, A., Nishina, S., Azuma, N., Kawahara, A., Kume, K., Nishina, H. (2019) The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish. Scientific Reports. 9:196
Kise, R., Okasato, R., Kano, K., Inoue, A., Kawahara, A., Aoki, J. (2019) Identification and biochemical characterization of a second zebrafish autotaxin gene. Journal of biochemistry. 165(3):269-275
Kawahara, A., Morita, H., Yanagi, K., Ishizaka, T., Taimatsu, K., Ohga, R. (2018) Developmental expression of the slurp-like1/ly2.3/ly97.3 and slurp-like2/ly2.2/ly97.2 genes during zebrafish early embryogenesis. Gene expression patterns : GEP. 30:32-36
Kawahara, A., Morita, H., Yanagi, K., Suzuki, H., Mori, T., Ohga, R., Taimatsu, K. (2018) Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis. Gene expression patterns : GEP. 30:1-6
Hino, H., Nakanishi, A., Seki, R., Aoki, T., Yamaha, E., Kawahara, A., Shimizu, T., Hibi, M. (2017) Roles of maternal wnt8a transcripts in axis formation in zebrafish. Developmental Biology. 434(1):96-107
Takeuchi, M., Inoue, C., Goshima, A., Nagao, Y., Shimizu, K., Miyamoto, H., Shimizu, T., Hashimoto, H., Yonemura, S., Kawahara, A., Hirata, Y., Yoshida, M., Hibi, M. (2017) Medaka and zebrafish contactin1 mutants as a model for understanding neural circuits for motor coordination. Genes to cells : devoted to molecular & cellular mechanisms. 22(8):723-741
Morita, H., Taimatsu, K., Yanagi, K., Kawahara, A. (2017) Exogenous gene integration mediated by genome editing technologies in zebrafish. Bioengineered. 8(3):287-295
Kawahara, A. (2017) CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1630:165-173
Ota, S., Taimatsu, K., Yanagi, K., Namiki, T., Ohga, R., Higashijima, S.I., Kawahara, A. (2016) Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish. Scientific Reports. 6:34991
Kawahara, A., Hisano, Y., Ota, S., Taimatsu, K. (2016) Site-Specific Integration of Exogenous Genes Using Genome Editing Technologies in Zebrafish. International Journal of Molecular Sciences. 17(5)
Ota, S., Kawahara, A. (2016) Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System. Methods in molecular biology (Clifton, N.J.). 1451:53-63
Mikami, S., Nakaura, M., Kawahara, A., Mizoguchi, T., Itoh, M. (2015) Mindbomb 2 is dispensable for embryonic development and Notch signalling in zebrafish. Biology Open. 4(11):1576-82
Stödberg, T., McTague, A., Ruiz, A.J., Hirata, H., Zhen, J., Long, P., Farabella, I., Meyer, E., Kawahara, A., Vassallo, G., Stivaros, S.M., Bjursell, M.K., Stranneheim, H., Tigerschiöld, S., Persson, B., Bangash, I., Das, K., Hughes, D., Lesko, N., Lundeberg, J., Scott, R.C., Poduri, A., Scheffer, I.E., Smith, H., Gissen, P., Schorge, S., Reith, M.E., Topf, M., Kullmann, D.M., Harvey, R.J., Wedell, A., Kurian, M.A. (2015) Mutations in SLC12A5 in epilepsy of infancy with migrating focal seizures. Nature communications. 6:8038
Hisano, Y., Inoue, A., Taimatsu, K., Ota, S., Ohga, R., Kotani, H., Muraki, M., Aoki, J., Kawahara, A. (2015) Comprehensive analysis of sphingosine-1-phosphate receptor mutants during zebrafish embryogenesis. Genes to cells : devoted to molecular & cellular mechanisms. 20(8):647-58
Hisano, Y., Inoue, A., Okudaira, M., Taimatsu, K., Matsumoto, H., Kotani, H., Ohga, R., Aoki, J., Kawahara, A. (2015) Maternal and Zygotic Sphingosine Kinase 2 are Indispensable for Cardiac Development in Zebrafish. The Journal of biological chemistry. 290(24):14841-51
Kotani, H., Taimatsu, K., Ohga, R., Ota, S., Kawahara, A. (2015) Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish. PLoS One. 10:e0128319
Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., Kawahara, A. (2015) Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Scientific Reports. 5:8841
Kimura, Y., Hisano, Y., Kawahara, A., Higashijima, S. (2014) Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering. Scientific Reports. 4:6545
Ota, S., Hisano, Y., Ikawa, Y., Kawahara, A. (2014) Multiple genome modifications by the CRISPR/Cas9 system in zebrafish. Genes to cells : devoted to molecular & cellular mechanisms. 19(7):555-64
Nakanaga, K., Hama, K., Kano, K., Sato, T., Yukiura, H., Inoue, A., Saigusa, D., Tokuyama, H., Tomioka, Y., Nishina, H., Kawahara, A., and Aoki, J. (2014) Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo. Journal of biochemistry. 155(4):235-41
Ota, S., and Kawahara, A. (2014) Zebrafish: a model vertebrate suitable for the analysis of human genetic disorders. Congenital anomalies. 54(1):8-11
Hisano, Y., Ota, S., and Kawahara, A. (2014) Genome editing using artificial site-specific nucleases in zebrafish. Development, growth & differentiation. 56(1):26-33
Iwasaki, R., Tsuge, K., Morimoto, K., Inazumi, T., Kawahara, O., Kawahara, A., Tsuchiya, S., and Sugimoto, Y. (2013) Molecular and pharmacological characterization of zebrafish 'contractile' and 'inhibitory' prostanoid receptors. Biochemical and Biophysical Research Communications. 438(2):353-8
Tsuge, K., Iwasaki, R., Morimoto, K., Inazumi, T., Kawahara, O., Kawahara, A., Tsuchiya, S., and Sugimoto, Y. (2013) Molecular and pharmacological characterization of zebrafish 'relaxant' prostanoid receptors. Biochemical and Biophysical Research Communications. 436(4):685-90
Hisano, Y., Ota, S., Takada, S., and Kawahara, A. (2013) Functional cooperation of spns2 and fibronectin in cardiac and lower jaw development. Biology Open. 2(8):789-794
Ota, S., Hisano, Y., Muraki, M., Hoshijima, K., Dahlem, T.J., Grunwald, D.J., Okada, Y., and Kawahara, A. (2013) Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays. Genes to cells : devoted to molecular & cellular mechanisms. 18(6):450-458
Hisano, Y., Ota, S., Arakawa, K., Muraki, M., Kono, N., Oshita, K., Sakuma, T., Tomita, M., Yamamoto, T., Okada, Y., and Kawahara, A. (2013) Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open. 2(4):363-367
Sato, Y., Mukai, M., Ueda, J., Muraki, M., Stasevich, T.J., Horikoshi, N., Kujirai, T., Kita, H., Kimura, T., Hira, S., Okada, Y., Hayashi-Takanaka, Y., Obuse, C., Kurumizaka, H., Kawahara, A., Yamagata, K., Nozaki, N., and Kimura, H. (2013) Genetically encoded system to track histone modification in vivo. Scientific Reports. 3:2436
Kawahara, A., Endo, S., and Dawid, I.B. (2012) Vap (Vascular Associated Protein): A novel factor involved in erythropoiesis and angiogenesis. Biochemical and Biophysical Research Communications. 421(2):367-374
Yukiura, H., Hama, K., Nakanaga, K., Tanaka, M., Asaoka, Y., Okudaira, S., Arima, N., Inoue, A., Hashimoto, T., Arai, H., Kawahara, A., Nishina, H., and Aoki, J. (2011) Autotaxin regulates vascular development via multiple lysophosphatidic acid (LPA) receptors in zebrafish. The Journal of biological chemistry. 286(51):43972-83
Iida, A., Sakaguchi, K., Sato, K., Sakurai, H., Nishimura, D., Iwaki, A., Takeuchi, M., Kobayashi, M., Misaki, K., Yonemura, S., Kawahara, A., and Sehara-Fujisawa, A. (2010) Metalloprotease-dependent onset of blood circulation in zebrafish. Current biology : CB. 20(12):1110-1116
Kawahara, A., and Stainier, D.Y. (2009) Noncanonical Activity of Seryl-Transfer RNA Synthetase and Vascular Development. Trends in cardiovascular medicine. 19(6):179-182
Fukui, H., Hanaoka, R., and Kawahara, A. (2009) Noncanonical Activity of Seryl-tRNA Synthetase Is Involved in Vascular Development. Circulation research. 104(11):1253-1259
Kitaguchi, T., Kawakami, K., and Kawahara, A. (2009) Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis. Mechanisms of Development. 126(5-6):314-323
Kawahara, A., Nishi, T., Hisano, Y., Fukui, H., Yamaguchi, A., and Mochizuki, N. (2009) The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors. Science (New York, N.Y.). 323(5913):524-527
Minehata, K.I., Kawahara, A., and Suzuki, T. (2008) meis1 regulates the development of endothelial cells in zebrafish. Biochemical and Biophysical Research Communications. 374(4):647-652
Hanaoka, R., Dawid, I.B., and Kawahara, A. (2007) Cloning and expression of zebrafish genes encoding the heme synthesis enzymes uroporphyrinogen III synthase (UROS) and protoporphyrinogen oxidase (PPO). DNA sequence : the journal of DNA sequencing and mapping. 18(1):54-60
Yamauchi, H., Hotta, Y., Konishi, M., Miyake, A., Kawahara, A., and Itoh, N. (2006) Fgf21 is essential for haematopoiesis in zebrafish. EMBO reports. 7(6):649-654
Hanaoka, R., Katayama, S., Dawid, I.B., and Kawahara, A. (2006) Characterization of the heme synthesis enzyme coproporphyrinogen oxidase (CPO) in zebrafish erythrogenesis. Genes to cells : devoted to molecular & cellular mechanisms. 11(3):293-303
Kawahara, A., Che, Y.S., Hanaoka, R., Takeda, H., and Dawid, I.B. (2005) Zebrafish GADD45{beta} genes are involved in somite segmentation. Proceedings of the National Academy of Sciences of the United States of America. 102(2):361-366
Kawahara, A. and Dawid, I.B. (2002) Developmental expression of zebrafish emx1 during early embryogenesis. Gene expression patterns : GEP. 2(3-4):201-206
Kawahara, A., Chien, C.-B., and Dawid, I.B. (2002) The homeobox gene mbx is involved in eye and tectum development. Developmental Biology. 248(1):107-117
Miyamoto, T., Kawahara, A., Teufel, A., Mukhopadhyay, M., Zhao, Y., Dawid, I.B., and Westphal, H. (2002) Mbx, a novel mouse homeobox gene. Development genes and evolution. 212(2):104-106
Kawahara, A. and Dawid, I.B. (2001) Critical role of biklf in erythroid cell differentiation in zebrafish. Current biology : CB. 11(17):1353-1357
Kawahara, A., Wilm, T., Solnica-Krezel, L., and Dawid, I.B. (2000) Antagonistic role of vega1 and bozozok/dharma homeobox genes in organizer formation. Proceedings of the National Academy of Sciences of the United States of America. 97(22):12121-12126
Kawahara, A., Wilm, T., Solnica-Krezel, L., and Dawid, I.B. (2000) Functional interaction of vega2 and goosecoid homeobox genes in zebrafish. Genesis (New York, N.Y. : 2000). 28(2):58-67
Kawahara, A. and Dawid, I.B. (2000) Expression of the Kruppel-like zinc finger gene biklf during zebrafish development. Mechanisms of Development. 97(1-2):173-176

NON-ZEBRAFISH PUBLICATIONS