ZFIN Person: Kawahara, Atsuo

ZFIN ID: ZDB-PERS-000914-13

Kawahara, Atsuo

Email:	akawahara@yamanashi.ac.jp
URL:
Affiliation:	Kawahara Lab
Address:	Laboratory for Developmental Biology Center for Medical Education and Sciences Graduate School of Medical Science University of Yamanashi Shimokato 1110, Chuo, Yamanashi, 409-3898, Japan
Country:	Japan
Phone:
Fax:
ORCID ID:

BIOGRAPHY AND RESEARCH INTERESTS

PUBLICATIONS

Suzuki, T., Hirai, Y., Uehara, T., Ohga, R., Kosaki, K., Kawahara, A. (2021) Involvement of the zebrafish trrap gene in craniofacial development. Scientific Reports. 11:24166

Sone, R., Taimatsu, K., Ohga, R., Nishimura, T., Tanaka, M., Kawahara, A. (2020) Critical roles of the ddx5 gene in zebrafish sex differentiation and oocyte maturation. Scientific Reports. 10:14157

Nguyen, V.T., Bian, L., Tamaoki, J., Otsubo, S., Muratani, M., Kawahara, A., Kobayashi, M. (2020) Generation and characterization of keap1a- and keap1b-knockout zebrafish. Redox Biology. 36:101667

Suzuki, H., Ishizaka, T., Yanagi, K., Sone, R., Sunaga, Y., Ohga, R., Kawahara, A. (2019) Characterization of biklf/klf17-deficient zebrafish in posterior lateral line neuromast and hatching gland development. Scientific Reports. 9:13680

Yanagi, K., Sone, R., Ohga, R., Kawahara, A. (2019) Involvement of the centrosomal protein 55 (cep55) gene in zebrafish head formation. Genes to cells : devoted to molecular & cellular mechanisms. 24(10):642-649

Iwasaki, R., Tsuge, K., Kishimoto, K., Hayashi, Y., Iwaana, T., Hohjoh, H., Inazumi, T., Kawahara, A., Tsuchiya, S., Sugimoto, Y. (2019) Essential role of prostaglandin E₂ and the EP3 receptor in lymphatic vessel development during zebrafish embryogenesis. Scientific Reports. 9:7650

Hirayama, J., Alifu, Y., Hamabe, R., Yamaguchi, S., Tomita, J., Maruyama, Y., Asaoka, Y., Nakahama, K.I., Tamaru, T., Takamatsu, K., Takamatsu, N., Hattori, A., Nishina, S., Azuma, N., Kawahara, A., Kume, K., Nishina, H. (2019) The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish. Scientific Reports. 9:196

Kise, R., Okasato, R., Kano, K., Inoue, A., Kawahara, A., Aoki, J. (2019) Identification and biochemical characterization of a second zebrafish autotaxin gene. Journal of biochemistry. 165(3):269-275

Kawahara, A., Morita, H., Yanagi, K., Ishizaka, T., Taimatsu, K., Ohga, R. (2018) Developmental expression of the slurp-like1/ly2.3/ly97.3 and slurp-like2/ly2.2/ly97.2 genes during zebrafish early embryogenesis. Gene expression patterns : GEP. 30:32-36

Kawahara, A., Morita, H., Yanagi, K., Suzuki, H., Mori, T., Ohga, R., Taimatsu, K. (2018) Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis. Gene expression patterns : GEP. 30:1-6

Hino, H., Nakanishi, A., Seki, R., Aoki, T., Yamaha, E., Kawahara, A., Shimizu, T., Hibi, M. (2017) Roles of maternal wnt8a transcripts in axis formation in zebrafish. Developmental Biology. 434(1):96-107

Takeuchi, M., Inoue, C., Goshima, A., Nagao, Y., Shimizu, K., Miyamoto, H., Shimizu, T., Hashimoto, H., Yonemura, S., Kawahara, A., Hirata, Y., Yoshida, M., Hibi, M. (2017) Medaka and zebrafish contactin1 mutants as a model for understanding neural circuits for motor coordination. Genes to cells : devoted to molecular & cellular mechanisms. 22(8):723-741

Morita, H., Taimatsu, K., Yanagi, K., Kawahara, A. (2017) Exogenous gene integration mediated by genome editing technologies in zebrafish. Bioengineered. 8(3):287-295

Kawahara, A. (2017) CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1630:165-173

Ota, S., Taimatsu, K., Yanagi, K., Namiki, T., Ohga, R., Higashijima, S.I., Kawahara, A. (2016) Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish. Scientific Reports. 6:34991

Kawahara, A., Hisano, Y., Ota, S., Taimatsu, K. (2016) Site-Specific Integration of Exogenous Genes Using Genome Editing Technologies in Zebrafish. International Journal of Molecular Sciences. 17(5)

Ota, S., Kawahara, A. (2016) Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System. Methods in molecular biology (Clifton, N.J.). 1451:53-63

Mikami, S., Nakaura, M., Kawahara, A., Mizoguchi, T., Itoh, M. (2015) Mindbomb 2 is dispensable for embryonic development and Notch signalling in zebrafish. Biology Open. 4(11):1576-82

Stödberg, T., McTague, A., Ruiz, A.J., Hirata, H., Zhen, J., Long, P., Farabella, I., Meyer, E., Kawahara, A., Vassallo, G., Stivaros, S.M., Bjursell, M.K., Stranneheim, H., Tigerschiöld, S., Persson, B., Bangash, I., Das, K., Hughes, D., Lesko, N., Lundeberg, J., Scott, R.C., Poduri, A., Scheffer, I.E., Smith, H., Gissen, P., Schorge, S., Reith, M.E., Topf, M., Kullmann, D.M., Harvey, R.J., Wedell, A., Kurian, M.A. (2015) Mutations in SLC12A5 in epilepsy of infancy with migrating focal seizures. Nature communications. 6:8038

Hisano, Y., Inoue, A., Taimatsu, K., Ota, S., Ohga, R., Kotani, H., Muraki, M., Aoki, J., Kawahara, A. (2015) Comprehensive analysis of sphingosine-1-phosphate receptor mutants during zebrafish embryogenesis. Genes to cells : devoted to molecular & cellular mechanisms. 20(8):647-58

Hisano, Y., Inoue, A., Okudaira, M., Taimatsu, K., Matsumoto, H., Kotani, H., Ohga, R., Aoki, J., Kawahara, A. (2015) Maternal and Zygotic Sphingosine Kinase 2 are Indispensable for Cardiac Development in Zebrafish. The Journal of biological chemistry. 290(24):14841-51

Kotani, H., Taimatsu, K., Ohga, R., Ota, S., Kawahara, A. (2015) Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish. PLoS One. 10:e0128319

Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., Kawahara, A. (2015) Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Scientific Reports. 5:8841

Kimura, Y., Hisano, Y., Kawahara, A., Higashijima, S. (2014) Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering. Scientific Reports. 4:6545

Ota, S., Hisano, Y., Ikawa, Y., Kawahara, A. (2014) Multiple genome modifications by the CRISPR/Cas9 system in zebrafish. Genes to cells : devoted to molecular & cellular mechanisms. 19(7):555-64

Nakanaga, K., Hama, K., Kano, K., Sato, T., Yukiura, H., Inoue, A., Saigusa, D., Tokuyama, H., Tomioka, Y., Nishina, H., Kawahara, A., and Aoki, J. (2014) Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo. Journal of biochemistry. 155(4):235-41

Ota, S., and Kawahara, A. (2014) Zebrafish: a model vertebrate suitable for the analysis of human genetic disorders. Congenital anomalies. 54(1):8-11

Hisano, Y., Ota, S., and Kawahara, A. (2014) Genome editing using artificial site-specific nucleases in zebrafish. Development, growth & differentiation. 56(1):26-33

Iwasaki, R., Tsuge, K., Morimoto, K., Inazumi, T., Kawahara, O., Kawahara, A., Tsuchiya, S., and Sugimoto, Y. (2013) Molecular and pharmacological characterization of zebrafish 'contractile' and 'inhibitory' prostanoid receptors. Biochemical and Biophysical Research Communications. 438(2):353-8

Tsuge, K., Iwasaki, R., Morimoto, K., Inazumi, T., Kawahara, O., Kawahara, A., Tsuchiya, S., and Sugimoto, Y. (2013) Molecular and pharmacological characterization of zebrafish 'relaxant' prostanoid receptors. Biochemical and Biophysical Research Communications. 436(4):685-90

Hisano, Y., Ota, S., Takada, S., and Kawahara, A. (2013) Functional cooperation of spns2 and fibronectin in cardiac and lower jaw development. Biology Open. 2(8):789-794

Ota, S., Hisano, Y., Muraki, M., Hoshijima, K., Dahlem, T.J., Grunwald, D.J., Okada, Y., and Kawahara, A. (2013) Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays. Genes to cells : devoted to molecular & cellular mechanisms. 18(6):450-458

Hisano, Y., Ota, S., Arakawa, K., Muraki, M., Kono, N., Oshita, K., Sakuma, T., Tomita, M., Yamamoto, T., Okada, Y., and Kawahara, A. (2013) Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open. 2(4):363-367

Sato, Y., Mukai, M., Ueda, J., Muraki, M., Stasevich, T.J., Horikoshi, N., Kujirai, T., Kita, H., Kimura, T., Hira, S., Okada, Y., Hayashi-Takanaka, Y., Obuse, C., Kurumizaka, H., Kawahara, A., Yamagata, K., Nozaki, N., and Kimura, H. (2013) Genetically encoded system to track histone modification in vivo. Scientific Reports. 3:2436

Kawahara, A., Endo, S., and Dawid, I.B. (2012) Vap (Vascular Associated Protein): A novel factor involved in erythropoiesis and angiogenesis. Biochemical and Biophysical Research Communications. 421(2):367-374

Yukiura, H., Hama, K., Nakanaga, K., Tanaka, M., Asaoka, Y., Okudaira, S., Arima, N., Inoue, A., Hashimoto, T., Arai, H., Kawahara, A., Nishina, H., and Aoki, J. (2011) Autotaxin regulates vascular development via multiple lysophosphatidic acid (LPA) receptors in zebrafish. The Journal of biological chemistry. 286(51):43972-83

Iida, A., Sakaguchi, K., Sato, K., Sakurai, H., Nishimura, D., Iwaki, A., Takeuchi, M., Kobayashi, M., Misaki, K., Yonemura, S., Kawahara, A., and Sehara-Fujisawa, A. (2010) Metalloprotease-dependent onset of blood circulation in zebrafish. Current biology : CB. 20(12):1110-1116

Kawahara, A., and Stainier, D.Y. (2009) Noncanonical Activity of Seryl-Transfer RNA Synthetase and Vascular Development. Trends in cardiovascular medicine. 19(6):179-182

Fukui, H., Hanaoka, R., and Kawahara, A. (2009) Noncanonical Activity of Seryl-tRNA Synthetase Is Involved in Vascular Development. Circulation research. 104(11):1253-1259

Kitaguchi, T., Kawakami, K., and Kawahara, A. (2009) Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis. Mechanisms of Development. 126(5-6):314-323

Kawahara, A., Nishi, T., Hisano, Y., Fukui, H., Yamaguchi, A., and Mochizuki, N. (2009) The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors. Science (New York, N.Y.). 323(5913):524-527

Minehata, K.I., Kawahara, A., and Suzuki, T. (2008) meis1 regulates the development of endothelial cells in zebrafish. Biochemical and Biophysical Research Communications. 374(4):647-652

Hanaoka, R., Dawid, I.B., and Kawahara, A. (2007) Cloning and expression of zebrafish genes encoding the heme synthesis enzymes uroporphyrinogen III synthase (UROS) and protoporphyrinogen oxidase (PPO). DNA sequence : the journal of DNA sequencing and mapping. 18(1):54-60

Yamauchi, H., Hotta, Y., Konishi, M., Miyake, A., Kawahara, A., and Itoh, N. (2006) Fgf21 is essential for haematopoiesis in zebrafish. EMBO reports. 7(6):649-654

Hanaoka, R., Katayama, S., Dawid, I.B., and Kawahara, A. (2006) Characterization of the heme synthesis enzyme coproporphyrinogen oxidase (CPO) in zebrafish erythrogenesis. Genes to cells : devoted to molecular & cellular mechanisms. 11(3):293-303

Kawahara, A., Che, Y.S., Hanaoka, R., Takeda, H., and Dawid, I.B. (2005) Zebrafish GADD45{beta} genes are involved in somite segmentation. Proceedings of the National Academy of Sciences of the United States of America. 102(2):361-366

Kawahara, A. and Dawid, I.B. (2002) Developmental expression of zebrafish emx1 during early embryogenesis. Gene expression patterns : GEP. 2(3-4):201-206

Kawahara, A., Chien, C.-B., and Dawid, I.B. (2002) The homeobox gene mbx is involved in eye and tectum development. Developmental Biology. 248(1):107-117

Miyamoto, T., Kawahara, A., Teufel, A., Mukhopadhyay, M., Zhao, Y., Dawid, I.B., and Westphal, H. (2002) Mbx, a novel mouse homeobox gene. Development genes and evolution. 212(2):104-106

Kawahara, A. and Dawid, I.B. (2001) Critical role of biklf in erythroid cell differentiation in zebrafish. Current biology : CB. 11(17):1353-1357

Kawahara, A., Wilm, T., Solnica-Krezel, L., and Dawid, I.B. (2000) Antagonistic role of vega1 and bozozok/dharma homeobox genes in organizer formation. Proceedings of the National Academy of Sciences of the United States of America. 97(22):12121-12126

Kawahara, A., Wilm, T., Solnica-Krezel, L., and Dawid, I.B. (2000) Functional interaction of vega2 and goosecoid homeobox genes in zebrafish. Genesis (New York, N.Y. : 2000). 28(2):58-67

Kawahara, A. and Dawid, I.B. (2000) Expression of the Kruppel-like zinc finger gene biklf during zebrafish development. Mechanisms of Development. 97(1-2):173-176

NON-ZEBRAFISH PUBLICATIONS