PUBLICATION
Automation of Technology for Cancer Research
- Authors
- van der Ent, W., Veneman, W.J., Groenewoud, A., Chen, L., Tulotta, C., Hogendoorn, P.C., Spaink, H.P., Snaar-Jagalska, B.E.
- ID
- ZDB-PUB-160512-18
- Date
- 2016
- Source
- Advances in experimental medicine and biology 916: 315-32 (Chapter)
- Registered Authors
- Snaar-Jagalska, Ewa B., Spaink, Herman P., van der Ent, Wietske
- Keywords
- Automation, Cancer, High-throughput, Image analysis, Microfluidics, Robotics, Zebrafish
- MeSH Terms
-
- Animals
- Automation*
- Disease Models, Animal*
- Microfluidics
- Microinjections
- Neoplasms/pathology*
- Zebrafish/embryology*
- PubMed
- 27165360 Full text @ Adv. Exp. Med. Biol.
Citation
van der Ent, W., Veneman, W.J., Groenewoud, A., Chen, L., Tulotta, C., Hogendoorn, P.C., Spaink, H.P., Snaar-Jagalska, B.E. (2016) Automation of Technology for Cancer Research. Advances in experimental medicine and biology. 916:315-32.
Abstract
Zebrafish embryos can be obtained for research purposes in large numbers at low cost and embryos develop externally in limited space, making them highly suitable for high-throughput cancer studies and drug screens. Non-invasive live imaging of various processes within the larvae is possible due to their transparency during development, and a multitude of available fluorescent transgenic reporter lines.To perform high-throughput studies, handling large amounts of embryos and larvae is required. With such high number of individuals, even minute tasks may become time-consuming and arduous. In this chapter, an overview is given of the developments in the automation of various steps of large scale zebrafish cancer research for discovering important cancer pathways and drugs for the treatment of human disease. The focus lies on various tools developed for cancer cell implantation, embryo handling and sorting, microfluidic systems for imaging and drug treatment, and image acquisition and analysis. Examples will be given of employment of these technologies within the fields of toxicology research and cancer research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping