PUBLICATION

Intracellular Golgi Complex Organization Reveals Tissue Specific Polarity during Zebrafish Embryogenesis

Authors
Sepich, D.S., Solnica-Krezel, L.
ID
ZDB-PUB-160405-1
Date
2016
Source
Developmental dynamics : an official publication of the American Association of Anatomists   245(6): 678-91 (Journal)
Registered Authors
Sepich, Diane, Solnica-Krezel, Lilianna
Keywords
GM130, Tbx16, Wnt/PCP, chordamesoderm, dispersed
MeSH Terms
  • Animals
  • Cell Polarity/genetics
  • Cell Polarity/physiology
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/genetics
  • Embryonic Development/physiology
  • Gastrulation/genetics
  • Gastrulation/physiology
  • Golgi Apparatus/genetics
  • Golgi Apparatus/metabolism*
  • Notochord/embryology
  • Notochord/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
27043944 Full text @ Dev. Dyn.
Abstract
Cell polarity is essential for directed migration of mesenchymal cells and morphogenesis of epithelial tissues. Studies in cultured cells indicate that a condensed Golgi Complex (GC) is essential for directed protein trafficking to establish cell polarity underlying directed cell migration. Dynamic changes of the GC intracellular organization during early vertebrate development remain to be investigated.
We used antibody labeling and fusion proteins in vivo to study the organization and intracellular placement of the GC during early zebrafish embryogenesis. We found that the GC was dispersed into several puncta containing cis- and trans-Golgi Complex proteins, presumably ministacks, until the end of the gastrula period. By early segmentation stages, the GC condensed in cells of the notochord, adaxial mesoderm and neural plate and its intracellular position became markedly polarized away from borders between these tissues.
We find that GC is dispersed in early zebrafish cells, even when cells are engaged in massive gastrulation movements. The GC accumulates into patches in a stage and cell-type specific manner, and becomes polarized away from borders between the embryonic tissues. With respect to tissue borders, intracellular GC polarity in notochord is independent of mature apical/basal polarity, Wnt/PCP or signals from adaxial mesoderm. This article is protected by copyright. All rights reserved.
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Errata and Notes