header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-160325-1
Sensitive red protein calcium indicators for imaging neural activity
Dana, H., Mohar, B., Sun, Y., Narayan, S., Gordus, A., Hasseman, J.P., Tsegaye, G., Holt, G.T., Hu, A., Walpita, D., Patel, R., Macklin, J.J., Bargmann, C.I., Ahrens, M.B., Schreiter, E.R., Jayaraman, V., Looger, L.L., Svoboda, K., Kim, D.S.
Date: 2016
Source: eLIFE   5: (Journal)
Registered Authors: Ahrens, Misha, Narayan, Sujatha, Sun, Yi
Keywords: C. elegans, D. melanogaster, mouse, neuroscience, zebrafish, calcium imaging, fluorescent probes, GECI, protein engineering
MeSH Terms:
  • Animals
  • Biosensing Techniques/methods*
  • Caenorhabditis elegans
  • Calcium/analysis*
  • Cells, Cultured
  • Drosophila
  • Intravital Microscopy/methods*
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism*
  • Mice
  • Neurons/chemistry*
  • Neurons/physiology*
  • Neurophysiology/methods*
  • Zebrafish
PubMed: 27011354 Full text @ Elife
ABSTRACT
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.
ADDITIONAL INFORMATION