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ZIRC
ZFIN ID: ZDB-PUB-160315-5
Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy
Quirin, S., Vladimirov, N., Yang, C.T., Peterka, D.S., Yuste, R., Ahrens, M. B.
Date: 2016
Source: Optics letters   41: 855-858 (Journal)
Registered Authors: Ahrens, Misha, Vladimirov, Nikita, Yang, Chao-Tsung
Keywords: none
MeSH Terms:
  • Animals
  • Brain/cytology
  • Caenorhabditis elegans
  • Calcium/metabolism*
  • Light*
  • Microscopy/methods*
  • Molecular Imaging
  • Neurons/metabolism*
PubMed: 26974063 Full text @ Opt. Lett.
ABSTRACT
Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160  μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.
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