|ZFIN ID: ZDB-PUB-160315-5|
Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy
Quirin, S., Vladimirov, N., Yang, C.T., Peterka, D.S., Yuste, R., Ahrens, M. B.
|Source:||Optics letters 41: 855-858 (Journal)|
|Registered Authors:||Ahrens, Misha, Vladimirov, Nikita, Yang, Chao-Tsung|
|PubMed:||26974063 Full text @ Opt. Lett.|
Quirin, S., Vladimirov, N., Yang, C.T., Peterka, D.S., Yuste, R., Ahrens, M. B. (2016) Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy. Optics letters. 41:855-858.
ABSTRACTIncreasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160 μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.