PUBLICATION

The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter

Authors
Suppiger, A., Eshwar, A.K., Stephan, R., Kaever, V., Eberl, L., Lehner, A.
ID
ZDB-PUB-160105-4
Date
2016
Source
Scientific Reports   6: 18753 (Journal)
Registered Authors
Keywords
Microbiology, Pathogens
MeSH Terms
  • Cronobacter/pathogenicity
  • Cronobacter/physiology*
  • Peptide Hydrolases/biosynthesis
  • Quorum Sensing*
  • Zebrafish
  • Bacterial Proteins/biosynthesis
  • Biofilms
  • Enterobacteriaceae Infections/microbiology
  • Animals
  • Biosensing Techniques
  • Cyclic GMP/analogs & derivatives
  • Cyclic GMP/metabolism
  • Phenotype
(all 13)
PubMed
26725701 Full text @ Sci. Rep.
Abstract
Several bacterial pathogens produce diffusible signal factor (DSF)-type quorum sensing (QS) signals to control biofilm formation and virulence. Previous work showed that in Burkholderia cenocepacia the RpfFBc/RpfR system is involved in sensing and responding to DSF signals and that this signal/sensor gene pair is highly conserved in several bacterial species including Cronobacter spp. Here we show that C. turicensis LMG 23827(T) possesses a functional RpfF/R system that is involved in the regulation of various phenotypes, including colony morphology, biofilm formation and swarming motility. In vivo experiments using the zebrafish embryo model revealed a role of this regulatory system in virulence of this opportunistic pathogen. We provide evidence that the RpfF/R system modulates the intracellular c-di-GMP level of the organism, an effect that may underpin the alteration in phenotype and thus the regulated phenotypes may be a consequence thereof. This first report on an RpfF/R-type QS system of an organism outside the genus Burkholderia revealed that both the underlying molecular mechanisms as well as the regulated functions show a high degree of conservation.
Genes / Markers
Figures
No images available
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
slc45a2b4/b4bacterial treatment by injection: Cronobacter turicensisProtruding-mouth
slc45a2b4/b4bacterial treatment by injection: Cronobacter turicensisDay 4
slc45a2b4/b4bacterial treatment by injection: Cronobacter turicensisDay 5
1 - 3 of 3
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b4
    		Insertion
    1 - 1 of 1
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    Human Disease / Model
    Sequence Targeting Reagents
    Fish
    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Suppiger, A., Eshwar, A.K., Stephan, R., Kaever, V., Eberl, L., Lehner, A. (2016) The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter. Scientific Reports. 6:18753.