PUBLICATION
Visualizing human Hematopoietic stem cell trafficking in vivo using a zebrafish xenograft model
- Authors
- Staal, F., Spaink, H., Fibbe, W.E.
- ID
- ZDB-PUB-151210-2
- Date
- 2016
- Source
- Stem cells and development 25(4): 360-5 (Journal)
- Registered Authors
- Spaink, Herman P.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation*
- Cell Lineage
- Cell Movement*
- Cell Tracking/methods*
- Embryo, Nonmammalian/cytology
- Hematopoietic Stem Cell Transplantation*
- Hematopoietic Stem Cells/cytology*
- Heterografts/cytology*
- Humans
- Models, Biological
- Stromal Cells/metabolism
- Zebrafish/metabolism*
- PubMed
- 26650921 Full text @ Stem Cells Dev.
Citation
Staal, F., Spaink, H., Fibbe, W.E. (2016) Visualizing human Hematopoietic stem cell trafficking in vivo using a zebrafish xenograft model. Stem cells and development. 25(4):360-5.
Abstract
Zebrafish is gaining increased popularity as a model organism to study stem cell biology. It also is widely used as model system to visualize human leukemic stem cells. However, xeno transplantation of primary human stem/progenitor cells has not been described. Here we use casper pigmentation mutant fish that are transparent crossed to fli-GFP transgenic fish as recipients of red labelled human CD34+ cells. We have investigated various conditions and protocols with the aim to monitor and visualize the fate of transplanted human CD34+ cells. We here report successful use of casper mutant zebrafish embryos for the direct monitoring of human HSC transplantation, differentiation and trafficking in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping