PUBLICATION

Zebrafish Egg Infection Model for Studying Candida albicans Adhesion Factors

Authors
Chen, Y.Z., Yang, Y.L., Chu, W.L., You, M.S., Lo, H.J.
ID
ZDB-PUB-151117-3
Date
2015
Source
PLoS One   10: e0143048 (Journal)
Registered Authors
You, May-su
Keywords
Candida albicans, Embryos, Zebrafish, Chorion, Biofilms, Inverted microscopy, Yeast infections, Mutant strains
MeSH Terms
  • Animals
  • Biofilms
  • Candida albicans/physiology*
  • Candidiasis/microbiology*
  • Embryo, Nonmammalian/microbiology
  • Hyphae/physiology
  • Ovum/microbiology*
  • Tissue Culture Techniques
  • Zebrafish
PubMed
26569623 Full text @ PLoS One
Abstract
Disseminated candidiasis is associated with 30-40% mortality in severely immunocompromised patients. Among the causal agents, Candida albicans is the dominant one. Various animal models have been developed for investigating gene functions in C. albicans. Zebrafish injection models have increasingly been applied in elucidating C. albicans pathogenesis because of the conserved immunity, prolific fecundity of the zebrafish and the low costs of care systems. In this study, we established a simple, noninvasive zebrafish egg bath infection model, defined its optimal conditions, and evaluated the model with various C. albicans mutant strains. The deletion of SAP6 did not have significant effect on the virulence. By contrast, the deletion of BCR1, CPH1, EFG1, or TEC1 significantly reduced the virulence under current conditions. Furthermore, all embryos survived when co-incubated with bcr1/bcr1, cph1/cph1 efg1/efg1, efg1/efg1, or tec1/tec1 mutant cells. The results indicated that our novel zebrafish model is time-saving and cost effective.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping