PUBLICATION
X-ray phase-contrast tomography for high-spatial-resolution zebrafish muscle imaging
- Authors
- Vågberg, W., Larsson, D.H., Li, M., Arner, A., Hertz, H.M.
- ID
- ZDB-PUB-151114-4
- Date
- 2015
- Source
- Scientific Reports 5: 16625 (Journal)
- Registered Authors
- Li, Mei
- Keywords
- Biomedical engineering, X-ray tomography
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Dystrophin/deficiency
- Dystrophin/genetics
- Imaging, Three-Dimensional/methods
- Larva/genetics
- Larva/metabolism
- Microscopy, Confocal
- Microscopy, Phase-Contrast
- Muscles/diagnostic imaging*
- Muscular Dystrophy, Animal/diagnostic imaging
- Muscular Dystrophy, Animal/genetics
- Muscular Dystrophy, Duchenne/diagnostic imaging
- Muscular Dystrophy, Duchenne/genetics
- Myofibrils/diagnostic imaging*
- Radiographic Image Enhancement/instrumentation
- Radiographic Image Enhancement/methods*
- Reproducibility of Results
- Tomography, X-Ray Computed/instrumentation
- Tomography, X-Ray Computed/methods*
- Zebrafish
- PubMed
- 26564785 Full text @ Sci. Rep.
Citation
Vågberg, W., Larsson, D.H., Li, M., Arner, A., Hertz, H.M. (2015) X-ray phase-contrast tomography for high-spatial-resolution zebrafish muscle imaging. Scientific Reports. 5:16625.
Abstract
Imaging of muscular structure with cellular or subcellular detail in whole-body animal models is of key importance for understanding muscular disease and assessing interventions. Classical histological methods for high-resolution imaging methods require excision, fixation and staining. Here we show that the three-dimensional muscular structure of unstained whole zebrafish can be imaged with sub-5 μm detail with X-ray phase-contrast tomography. Our method relies on a laboratory propagation-based phase-contrast system tailored for detection of low-contrast 4-6 μm subcellular myofibrils. The method is demonstrated on 20 days post fertilization zebrafish larvae and comparative histology confirms that we resolve individual myofibrils in the whole-body animal. X-ray imaging of healthy zebrafish show the expected structured muscle pattern while specimen with a dystrophin deficiency (sapje) displays an unstructured pattern, typical of Duchenne muscular dystrophy. The method opens up for whole-body imaging with sub-cellular detail also of other types of soft tissue and in different animal models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping