ZFIN ID: ZDB-PUB-151110-4
Temporal and organ-specific detection of cNMPs including cUMP in the zebrafish
Dittmar, F., Abdelilah-Seyfried, S., Tschirner, S.K., Kaever, V., Seifert, R.
Date: 2015
Source: Biochemical and Biophysical Research Communications   468(4): 708-12 (Journal)
Registered Authors: Abdelilah-Seyfried, Salim
Keywords: cyclic CMP, cyclic UMP, zebrafish
MeSH Terms:
  • Aging/physiology
  • Animals
  • Cyclic CMP/metabolism*
  • Embryonic Development/physiology*
  • Gene Expression Regulation, Developmental/physiology
  • Nucleotides, Cyclic/metabolism*
  • Organ Specificity/physiology
  • Tissue Distribution
  • Uridine Monophosphate/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
PubMed: 26551461 Full text @ Biochem. Biophys. Res. Commun.
The cyclic pyrimidine nucleotides cCMP and cUMP occur in mammalian cell lines. Recently, cCMP was also identified in mouse organs. Due to technical difficulties, it has not been possible to detect cUMP in organs or tissues yet. Here, we have generated a temporal profile of the occurrence of nucleoside 3',5'-cyclic monophosphates during different developmental stages of embryogenesis and in different organs of the adult zebrafish Danio rerio. Cyclic nucleotides were quantified by high performance liquid chromatography quadrupole tandem mass spectrometry. The identity of cCMP and cUMP in the zebrafish was confirmed by high performance liquid chromatography quadrupole time-of-flight mass spectrometry. We show for the first time that cUMP can be detected during embryogenesis and in adult organs of this vertebrate model system.