PUBLICATION
A Kupffer's vesicle size threshold for robust left-right patterning of the zebrafish embryo
- Authors
- Gokey, J.J., Ji, Y., Tay, H.G., Litts, B., Amack, J.D.
- ID
- ZDB-PUB-151008-9
- Date
- 2016
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 245(1): 22-33 (Journal)
- Registered Authors
- Amack, Jeffrey, Gokey, Jason, Ji, Yongchang, Tay, Hwee Goon
- Keywords
- Cilia, Congenital disease, Developmental noise, Left-right patterning, Organ size control
- MeSH Terms
-
- Animals
- Body Patterning/physiology*
- Cilia/physiology
- Embryo, Nonmammalian/physiology
- Embryonic Development/physiology*
- Zebrafish
- PubMed
- 26442502 Full text @ Dev. Dyn.
Citation
Gokey, J.J., Ji, Y., Tay, H.G., Litts, B., Amack, J.D. (2016) A Kupffer's vesicle size threshold for robust left-right patterning of the zebrafish embryo. Developmental Dynamics : an official publication of the American Association of Anatomists. 245(1):22-33.
Abstract
Background Motile cilia in the 'organ of asymmetry' create directional fluid flows that are vital for left-right (LR) asymmetric patterning of vertebrate embryos. Organ function often depends on tightly regulated organ size control, but the role of organ of asymmetry size in LR patterning has remained unknown. Observations of the organ of asymmetry in the zebrafish -called Kupffer's vesicle (KV)-have suggested significant variations in KV size in wild-type embryos, raising questions about the impact of KV organ size on LR patterning.
Results To understand the relationship between organ of asymmetry size and its function, we characterized variations in KV at several developmental stages and in several different zebrafish strains. We found that the number of KV cilia and the size of the KV lumen were highly variable, whereas the length of KV cilia showed less variation. These variabilities were similar among different genetic backgrounds. By specifically modulating KV size and analyzing individual embryos, we identified a size threshold that is necessary for KV function.
Conclusions Together these results indicate the KV organ of asymmetry size is not tightly controlled during development, but rather must only exceed a threshold to direct robust LR patterning of the zebrafish embryo. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping