PUBLICATION
A zebrafish jam-b2 Gal4-enhancer trap line recapitulates endogenous jam-b2 expression in extraocular muscles
- Authors
- Matsui, H., Dorigo, A., Buchberger, A., Hocking, J.C., Distel, M., Köster, R.W.
- ID
- ZDB-PUB-150916-3
- Date
- 2015
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 244(12): 1574-80 (Journal)
- Registered Authors
- Distel, Martin, Hocking, Jennifer, Köster, Reinhard W., Matsui, Hideaki
- Keywords
- Extraocular muscle, Junctional adhesion molecule, Zebrafish
- MeSH Terms
-
- Animal Fins/embryology
- Animal Fins/metabolism*
- Animals
- Animals, Genetically Modified
- Gene Expression Regulation, Developmental
- Junctional Adhesion Molecule B/genetics
- Junctional Adhesion Molecule B/metabolism*
- Muscle, Skeletal/embryology
- Muscle, Skeletal/metabolism*
- Zebrafish/embryology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 26370768 Full text @ Dev. Dyn.
Citation
Matsui, H., Dorigo, A., Buchberger, A., Hocking, J.C., Distel, M., Köster, R.W. (2015) A zebrafish jam-b2 Gal4-enhancer trap line recapitulates endogenous jam-b2 expression in extraocular muscles. Developmental Dynamics : an official publication of the American Association of Anatomists. 244(12):1574-80.
Abstract
Background Members of the Junctional Adhesion Molecule (JAM) family function as cell adhesion molecules and cell surface receptors. The zebrafish genome contains six different jam genes, and jam-b and jam-c were shown to be essential for myoblast fusion during skeletal muscle development. However little is known about jam-b2 expression and function.
Results We isolated the cDNA of zebrafish jam-b2. jam-b2 is expressed specifically in EOMs, jaw muscles and pectoral fins in zebrafish larvae, but not in trunk muscles. The identified jam-b2 expression pattern is supported by the analysis of a zebrafish Gal4-enhancer trap line, in which the coding sequence of the transcriptional activator KalTA4 together with a Gal4-dependent UAS-mCherry expression cassette was inserted into the jam-b2 locus. Intercrosses with an UAS:EGFP strain proves the possibility for targeting transgene expression to EOMs, jaw muscles and fins. Finally, we characterized the concerted contraction pattern of EOMs in larvae performing an optokinetic response.
Conclusions The expression pattern of jam-b2 suggests that it may contribute different properties to EOMs, jaw muscles and pectoral fins. The jam-b2:KalTA4-UAS-mCherry transgenic strain serves a dual role as both a reporter for these muscles and as a valuable genetic tool for targeting transgene expression to EOMs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping