PUBLICATION
Rapid high performance liquid chromatography-high resolution mass spectrometry methodology for multiple prenol lipids analysis in zebrafish embryos
- Authors
- Martano, C., Mugoni, V., Dal Bello, F., Santoro, M.M., Medana, C.
- ID
- ZDB-PUB-150819-4
- Date
- 2015
- Source
- Journal of chromatography. A 1412: 59-66 (Journal)
- Registered Authors
- Mugoni, Vera, Santoro, Massimo
- Keywords
- C4 reverse phase, HPLC–HRMS, Prenol lipids, Zebrafish
- MeSH Terms
-
- Animals
- Cholesterol/analysis
- Chromatography, High Pressure Liquid/methods
- Embryo, Nonmammalian/metabolism
- Mass Spectrometry/methods
- Mutation
- Pentanols/analysis*
- Ubiquinone/analysis
- Vitamin E/analysis
- Vitamin K 1/analysis
- Vitamin K 2/analysis
- Zebrafish/genetics
- Zebrafish/metabolism*
- alpha-Tocopherol/analysis
- PubMed
- 26283533 Full text @ J. Chromatogr. A
Citation
Martano, C., Mugoni, V., Dal Bello, F., Santoro, M.M., Medana, C. (2015) Rapid high performance liquid chromatography-high resolution mass spectrometry methodology for multiple prenol lipids analysis in zebrafish embryos. Journal of chromatography. A. 1412:59-66.
Abstract
The analysis of lipid molecules in living organism is an important step in deciphering metabolic pathways. Recently, the zebrafish has been adopted as a valuable animal model system to perform in vivo metabolomics studies, however limited methodologies and protocols are currently available to investigate zebrafish lipidome and even fewer to analyze specific classes of lipids. Here we present an HPLC-HRMS based method to rapidly measure multiple prenol lipid molecules from zebrafish tissues. In particular, we have optimized our method for concurrent detection of ubiquinones (Coenzyme Q6, Coenzyme Q9, Coenzyme Q10), cholesterol, vitamin E (α-tocopherol), vitamin K1 and vitamin K2. The purpose of this study was to compare different ionization modes, mobile phases and stationary phases in order to optimize lipid molecules separation. After HPLC-HRMS parameters selection, several extraction conditions from zebrafish embryos were evaluated. We assessed our methodology by quantitation of analytical recovery on zebrafish extracts from wild-type or zebrafish mutants (barolo) affected by impaired biosynthesis of ubiquinones.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping