PUBLICATION

Identification and expression of Lypc, a novel dark-inducible member of Ly6 superfamily in zebrafish Danio rerio

Authors

Li, L., Ji, D., Teng, L., Zhang, S., Li, H.

ID

ZDB-PUB-150819-14

Date

2015

Source

Gene 574(1): 69-75 (Journal)

Registered Authors

Li, Hongyan

Keywords

Ly6 superfamily, Lypc, pigment cells, zebrafish

MeSH Terms

Animals
Chromosomes/genetics
Gene Expression/genetics*
Gene Expression Regulation, Developmental/genetics
Lymphocyte Antigen 96/genetics*
Zebrafish/genetics*
Zebrafish Proteins/genetics*

(all 7)

PubMed

26278158 Full text @ Gene

Abstract

Snake venom neurotoxins and lymphocyte antigen-6 (Ly6) family members identified in many metazoans possess conserved LU domain containing eight or ten conserved cysteine residues which form 4 to 5 conserved disulfide bonds. They are found to execute a wide variety of biological effects, but information regarding Ly6 superfamily in zebrafish remains rather limited. Here we identified a novel Ly6 gene located on the chromosome 15 in zebrafish, and named it Lypc highlighting its predominant expression in the pigment cells. Both homology modeling and sequence comparison revealed that Lypc has features typical of Ly6 family members. Whole mount in situ hybridization showed that Lypc was expressed in the trunk pigment cells and retinal pigment cells. Moreover, real-time quantitative PCR demonstrated that the expression of Lypc was subjected to diurnal variation regulation, i.e. the expression of Lypc displays a clear rhythmic pattern, and dark exposure apparently stimulated its expression. Collectively, these data indicate that Lypc is a novel dark-inducible Ly6 member with a predominant expression in the pigment cells of zebrafish, laying a foundation for further elucidation of its functions.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
lypc	mitfa^w2/w2; mpv17^a9/a9	standard conditions	Day 4	retina	ISH	Fig. 5
lypc	mitfa^w2/w2; mpv17^a9/a9	standard conditions	Day 5	retina	ISH	Fig. 5
lypc	mitfa^w2/w2; mpv17^a9/a9	standard conditions	Long-pec	retina	ISH	Fig. 5
lypc	mitfa^w2/w2; mpv17^a9/a9	standard conditions	Protruding-mouth	retina	ISH	Fig. 5
lypc	WT	standard conditions	Protruding-mouth	dorsal larval melanophore stripe	ISH	Fig. 3
lypc	WT	standard conditions	Protruding-mouth	epidermis pigment cell	ISH	Fig. 3
lypc	WT	standard conditions	Adult	eye	RTPCR	Fig. 2
lypc	WT	standard conditions	Adult	heart	RTPCR	Fig. 2
lypc	WT	standard conditions	Adult	integument	RTPCR	Fig. 2
lypc	WT	standard conditions	Adult	intestine	RTPCR	Fig. 2

1 - 10 of 31

Show

Phenotype

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
a9		Complex	mpv17
w2		Point Mutation	mitfa

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
mitfa^w2/w2; mpv17^a9/a9
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Li et al., 2015 ZDB-PUB-150819-14 PMID:26278158

Identification and expression of Lypc, a novel dark-inducible member of Ly6 superfamily in zebrafish Danio rerio

Li et al., 2015

ZDB-PUB-150819-14
PMID:26278158