PUBLICATION
Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
- Authors
- Kamei, C.N., Liu, Y., Drummond, I.A.
- ID
- ZDB-PUB-150815-2
- Date
- 2015
- Source
- Journal of visualized experiments : JoVE (102): e51912 (Journal)
- Registered Authors
- Drummond, Iain, Liu, Yan
- Keywords
- Developmental biology, kidney, mesonephros, gentamicin, injection, zebrafish, injury, regeneration, Lhx1a, heat shock, adult
- MeSH Terms
-
- Gentamicins/administration & dosage*
- Male
- Nephrons/drug effects
- Nephrons/physiology
- Acute Kidney Injury/chemically induced*
- Regeneration/drug effects*
- Regeneration/physiology*
- Stem Cells/drug effects
- Stem Cells/physiology
- Female
- Kidney/drug effects*
- Kidney/physiology*
- Injections, Intraperitoneal
- Animals
- Zebrafish/physiology*
- PubMed
- 26275011 Full text @ J. Vis. Exp.
Citation
Kamei, C.N., Liu, Y., Drummond, I.A. (2015) Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury. Journal of visualized experiments : JoVE. (102):e51912.
Abstract
The kidney is essential for fluid homeostasis, blood pressure regulation and filtration of waste from the body. The fundamental unit of kidney function is the nephron. Mammals are able to repair existing nephrons after injury, but lose the ability to form new nephrons soon after birth. In contrast to mammals, adult fish produce new nephrons (neonephrogenesis) throughout their lives in response to growth requirements or injury. Recently, lhx1a has been shown to mark nephron progenitor cells in the adult zebrafish kidney, however mechanisms controlling the formation of new nephrons after injury remain unknown. Here we show our method for robust and reproducible injury in the adult zebrafish kidney by intraperitoneal (i.p.) injection of gentamicin, which uses a noninvasive visual screening process to select for fish with strong but nonlethal injury. Using this method, we can determine optimal gentamicin dosages for injury and go on to demonstrate the effect of higher temperatures on kidney regeneration in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping