ZFIN ID: ZDB-PUB-150808-13
Recoverin depletion accelerates cone photoresponse recovery
Zang, J., Keim, J., Kastenhuber, E., Gesemann, M., Neuhauss, S.C.
Date: 2015
Source: Open Biology   5(8): (Journal)
Registered Authors: Gesemann, Matthias, Kastenhuber, Edda, Neuhauss, Stephan, Zang, Jingjing
Keywords: cone photoreceptor, electroretinogram, phototransduction termination, zebrafish
MeSH Terms:
  • Animals
  • G-Protein-Coupled Receptor Kinases/metabolism
  • Gene Expression
  • Gene Knockdown Techniques
  • Molecular Sequence Data
  • Organ Specificity/genetics
  • Recoverin/deficiency
  • Recoverin/genetics
  • Recoverin/metabolism*
  • Retinal Cone Photoreceptor Cells/physiology*
  • Retinal Cone Photoreceptor Cells/radiation effects
  • Ultraviolet Rays
  • Zebrafish
  • Zebrafish Proteins
PubMed: 26246494 Full text @ Open Biol.
The neuronal Ca(2+)-binding protein Recoverin has been shown to regulate phototransduction termination in mammalian rods. Here we identify four recoverin genes in the zebrafish genome, rcv1a, rcv1b, rcv2a and rcv2b, and investigate their role in modulating the cone phototransduction cascade. While Recoverin-1b is only found in the adult retina, the other Recoverins are expressed throughout development in all four cone types, except Recoverin-1a, which is expressed only in rods and UV cones. Applying a double flash electroretinogram (ERG) paradigm, downregulation of Recoverin-2a or 2b accelerates cone photoresponse recovery, albeit at different light intensities. Exclusive recording from UV cones via spectral ERG reveals that knockdown of Recoverin-1a alone has no effect, but Recoverin-1a/2a double-knockdowns showed an even shorter recovery time than Recoverin-2a-deficient larvae. We also showed that UV cone photoresponse kinetics depend on Recoverin-2a function via cone-specific kinase Grk7a. This is the first in vivo study demonstrating that cone opsin deactivation kinetics determine overall photoresponse shut off kinetics.