PUBLICATION
Identification of polarized macrophage subsets in zebrafish
- Authors
- Nguyen Chi, M., Laplace-Builhe, B., Travnickova, J., Luz-Crawford, P., Tejedor, G., Phan, Q.T., Duroux-Richard, I., Levraud, J.P., Kissa, K., Lutfalla, G., Jorgensen, C., Djouad, F.
- ID
- ZDB-PUB-150711-11
- Date
- 2015
- Source
- eLIFE 4: e07288 (Journal)
- Registered Authors
- Djouad, Farida, Kissa-Marin, Karima, Levraud, Jean-Pierre, Lutfalla, Georges, Nguyen-Chi, Mai, Phan, Quang Tien
- Keywords
- developmental biology, immunology, live imaging, macrophages, stem cells, zebrafish
- MeSH Terms
-
- Macrophages/classification*
- Macrophages/immunology*
- Molecular Sequence Data
- Tumor Necrosis Factor-alpha/biosynthesis
- Sequence Analysis, DNA
- Gene Expression Profiling
- Genes, Reporter
- Flow Cytometry
- Wounds and Injuries/immunology
- Real-Time Polymerase Chain Reaction
- Zebrafish/immunology*
- Escherichia coli Infections/immunology
- Animals, Genetically Modified
- Animals
- Microscopy, Confocal
- Reverse Transcriptase Polymerase Chain Reaction
- PubMed
- 26154973 Full text @ Elife
Citation
Nguyen Chi, M., Laplace-Builhe, B., Travnickova, J., Luz-Crawford, P., Tejedor, G., Phan, Q.T., Duroux-Richard, I., Levraud, J.P., Kissa, K., Lutfalla, G., Jorgensen, C., Djouad, F. (2015) Identification of polarized macrophage subsets in zebrafish. eLIFE. 4:e07288.
Abstract
While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tnfa, a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and E. coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on FACS-sorted tnfa+ and tnfa- macrophages showed that they respectively expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa+ macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping