PUBLICATION
Neural Differentiation Modulates the Vertebrate Brain Specific Splicing Program
- Authors
- Madgwick, A., Fort, P., Hanson, P.S., Thibault, P., Gaudreau, M.C., Lutfalla, G., Möröy, T., Abou Elela, S., Chaudhry, B., Elliott, D.J., Morris, C.M., Venables, J.P.
- ID
- ZDB-PUB-150521-4
- Date
- 2015
- Source
- PLoS One 10: e0125998 (Journal)
- Registered Authors
- Lutfalla, Georges
- Keywords
- none
- MeSH Terms
-
- Alternative Splicing*
- Animals
- Cell Differentiation*
- Humans
- Mice
- Neurons/cytology*
- Real-Time Polymerase Chain Reaction
- Vertebrates/metabolism*
- Zebrafish
- PubMed
- 25993117 Full text @ PLoS One
Citation
Madgwick, A., Fort, P., Hanson, P.S., Thibault, P., Gaudreau, M.C., Lutfalla, G., Möröy, T., Abou Elela, S., Chaudhry, B., Elliott, D.J., Morris, C.M., Venables, J.P. (2015) Neural Differentiation Modulates the Vertebrate Brain Specific Splicing Program. PLoS One. 10:e0125998.
Abstract
Alternative splicing patterns are known to vary between tissues but these patterns have been found to be predominantly peculiar to one species or another, implying only a limited function in fundamental neural biology. Here we used high-throughput RT-PCR to monitor the expression pattern of all the annotated simple alternative splicing events (ASEs) in the Reference Sequence Database, in different mouse tissues and identified 93 brain-specific events that shift from one isoform to another (switch-like) between brain and other tissues. Consistent with an important function, regulation of a core set of 9 conserved switch-like ASEs is highly conserved, as they have the same pattern of tissue-specific splicing in all vertebrates tested: human, mouse and zebrafish. Several of these ASEs are embedded within genes that encode proteins associated with the neuronal microtubule network, and show a dramatic and concerted shift within a short time window of human neural stem cell differentiation. Similarly these exons are dynamically regulated in zebrafish development. These data demonstrate that although alternative splicing patterns often vary between species, there is nonetheless a core set of vertebrate brain-specific ASEs that are conserved between species and associated with neural differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping