PUBLICATION

Developmental origins of neurotransmitter and transcriptome alterations in adult female zebrafish exposed to atrazine during embryogenesis

Authors
Wirbisky, S.E., Weber, G.J., Sepúlveda, M.S., Xiao, C., Cannon, J.R., Freeman, J.L.
ID
ZDB-PUB-150502-5
Date
2015
Source
Toxicology   333: 156-67 (Journal)
Registered Authors
Freeman, Jennifer
Keywords
atrazine, development, developmental origins of health and disease, neurotransmitters, transcriptomics, zebrafish
Datasets
GEO:GSE117260
MeSH Terms
  • Down-Regulation
  • Transcriptome/drug effects*
  • Dose-Response Relationship, Drug
  • Larva/drug effects
  • Larva/genetics
  • Larva/metabolism
  • Animals
  • Gene Expression Profiling/methods
  • Male
  • Gene Expression Regulation
  • Hydroxyindoleacetic Acid/metabolism*
  • Female
  • Time Factors
  • Water Pollutants, Chemical/toxicity*
  • Atrazine/toxicity*
  • Age Factors
  • Endocrine Disruptors/toxicity*
  • Serotonergic Neurons/drug effects*
  • Serotonergic Neurons/metabolism
  • Serotonin/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Risk Assessment
  • Herbicides/toxicity*
  • Sex Factors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Oligonucleotide Array Sequence Analysis
  • Brain/drug effects*
  • Brain/embryology
  • Brain/metabolism
(all 32)
PubMed
25929836 Full text @ Toxicology
CTD
25929836
Abstract
Atrazine is an herbicide applied to agricultural crops and is indicated to be an endocrine disruptor. Atrazine is frequently found to contaminate potable water supplies above the maximum contaminant level of 3μg/L as defined by the U. S. Environmental Protection Agency. The developmental origin of adult disease hypothesis suggests that toxicant exposure during development can increase the risk of certain diseases during adulthood. However, the molecular mechanisms underlying disease progression are still unknown. In this study, zebrafish embryos were exposed to 0, 0.3, 3, or 30μg/L atrazine throughout embryogenesis. Larvae were then allowed to mature under normal laboratory conditions with no further chemical treatment until 7 days post fertilization (dpf) or adulthood and neurotransmitter analysis completed. No significant alterations in neurotransmitter levels was observed at 7 dpf or in adult males, but a significant decrease in 5-Hydroxyindoleacetic acid (5-HIAA) and serotonin turnover was seen in adult female brain tissue. Transcriptomic analysis was completed on adult female brain tissue to identify molecular pathways underlying the observed neurological alterations. Altered expression of 1853, 84, and 419 genes in the females exposed to 0.3, 3, or 30μg/L atrazine during embryogenesis were identified, respectively. There was a high level of overlap between the biological processes and molecular pathways in which the altered genes were associated. Moreover, a subset of genes was down regulated throughout the serotonergic pathway. These results provide support of the developmental origins of neurological alterations observed in adult female zebrafish exposed to atrazine during embryogenesis.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Wirbisky, S.E., Weber, G.J., Sepúlveda, M.S., Xiao, C., Cannon, J.R., Freeman, J.L. (2015) Developmental origins of neurotransmitter and transcriptome alterations in adult female zebrafish exposed to atrazine during embryogenesis. Toxicology. 333:156-67.