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Research
General Information
ZIRC
ZFIN ID: ZDB-PERS-050519-26
Freeman, Jennifer
Email: jfreema@purdue.edu
URL: http://www.healthsciences.purdue.edu/people/faculty.php?uid=jfreema
Affiliation: Charles Lee's Molecular Cytogenetic Research Lab
and also: Freeman Lab
Address: Purdue University School of Health Sciences 550 Stadium Mall Dr. West Lafayette, IN 47907 USA
Country: United States
Phone: 765-494-1408
Fax: 765-496-1377
Orcid ID:


BIOGRAPHY AND RESEARCH INTERESTS
Jennifer L. Freeman is an Associate Professor in the School of Health Sciences at Purdue University in West Lafayette, IN. She received a B.S. in Cell and Structural Biology from the University of Illinois at Urbana-Champaign and a Ph.D. in Environmental Toxicology and Molecular Cytogenetics from the University of Illinois at Urbana-Champaign. She did her post-doctoral work at Harvard Medical School and Brigham and Women’s Hospital, Boston. The interests of the Freeman laboratory are to define the underlying genetic and epigenetic mechanisms of toxicity of environmental stressors with current focus on pesticides, metals, radiation, and emerging contaminants. Projects are defining the immediate adverse impacts of a developmental exposure, the lasting adverse impacts of this developmental exposure throughout the lifespan, and the analysis of subsequent generations linking genetic, epigenetic, and phenotypic assessments. These studies are investigating the developmental origin of health and disease pathogenesis with a specific focus on neurological disorders, reproductive dysfunction, cardiovascular function, and cancer with a goal of understanding the role of exposure to the environmental stressors in these adverse health outcomes. All projects are currently utilizing the zebrafish vertebrate model system as a tool to investigate toxicity.


PUBLICATIONS
Pitchai, A., Rajaretinam, R.K., Freeman, J.L. (2019) Zebrafish as an Emerging Model for Bioassay-Guided Natural Product Drug Discovery for Neurological Disorders. Medicines (Basel, Switzerland). 6(2)
Horzmann, K.A., Reidenbach, L.S., Thanki, D.H., Winchester, A.E., Qualizza, B.A., Ryan, G.A., Egan, K.E., Hedrick, V.E., Sobreira, T.J.P., Peterson, S.M., Weber, G.J., Wirbisky-Hershberger, S.E., Sepúlveda, M.S., Freeman, J.L. (2018) Embryonic atrazine exposure elicits proteomic, behavioral, and brain abnormalities with developmental time specific gene expression signatures. Journal of proteomics. 186:71-82
Caballero-Gallardo, K., Wirbisky-Hershberger, S.E., Olivero-Verbel, J., de la Rosa, J., Freeman, J.L. (2018) Embryonic exposure to an aqueous coal dust extract results in gene expression alterations associated with the development and function of connective tissue and the hematological system, immunological and inflammatory disease, and cancer in zebrafish. Metallomics : integrated biometal science. 10(3):463-473
Horzmann, K.A., Freeman, J.L. (2018) Making Waves: New Developments in Toxicology with the Zebrafish. Toxicological sciences : an official journal of the Society of Toxicology. 163(1):5-12
Zhou, W., Pal, A.S., Hsu, A.Y., Gurol, T., Zhu, X., Wirbisky-Hershberger, S.E., Freeman, J.L., Kasinski, A.L., Deng, Q. (2018) MicroRNA-223 Suppresses the Canonical NF-κB Pathway in Basal Keratinocytes to Dampen Neutrophilic Inflammation. Cell Reports. 22:1810-1823
Horzmann, K.A., de Perre, C., Lee, L.S., Whelton, A.J., Freeman, J.L. (2017) Comparative analytical and toxicological assessment of methylcyclohexanemethanol (MCHM) mixtures associated with the Elk River chemical spill. Chemosphere. 188:599-607
Wirbisky-Hershberger, S.E., Sanchez, O.F., Horzmann, K.A., Thanki, D., Yuan, C., Freeman, J.L. (2017) Atrazine exposure decreases the activity of DNMTs, global DNA methylation levels, and dnmt expression. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 109(Pt 1):727-734
Lee, J., Horzmann, K.A., Freeman, J.L. (2017) An embryonic 100μg/L lead exposure results in sex-specific expression changes in genes associated with the neurological system in female or cancer in male adult zebrafish brains. Neurotoxicology and teratology. 65:60-69
Damayanti, N.P., Buno, K., Cui, Y., Voytik-Harbin, S.L., Pili, R., Freeman, J., Irudayaraj, J.M.K. (2017) Real-Time Multiplex Kinase Phosphorylation Sensors in Living Cells. ACS sensors. 2:1225-1230
Elsayed, M.S.A., Su, Y., Wang, P., Sethi, T., Agama, K., Ravji, A., Redon, C.E., Kiselev, E., Horzmann, K.A., Freeman, J.L., Pommier, Y., Cushman, M. (2017) Design and Synthesis of Chlorinated and Fluorinated 7-Azaindenoisoquinolines as Potent Cytotoxic Anticancer Agents That Inhibit Topoisomerase I. Journal of medicinal chemistry. 60:5364-5376
Wirbisky, S.E., Freeman, J.L. (2017) Atrazine Exposure Elicits Copy Number Alterations in the Zebrafish Genome. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 194:1-8
Lee, J., Peterson, S.M., Freeman, J.L. (2017) Sex-specific characterization and evaluation of the Alzheimer's disease genetic risk factor sorl1 in zebrafish during aging and in the adult brain following a 100 ppb embryonic lead exposure. Journal of applied toxicology : JAT. 37(4):400-407
Sanchez, O.F., Lee, J., Yu King Hing, N., Kim, S.E., Freeman, J.L., Yuan, C. (2017) Lead (Pb) exposure reduces global DNA methylation level by non-competitive inhibition and alteration of dnmt expression. Metallomics : integrated biometal science. 9(2):149-160
Wirbisky, S.E., Weber, G.J., Schlotman, K.E., Sepúlveda, M.S., Freeman, J.L. (2016) Embryonic atrazine exposure alters zebrafish and human miRNAs associated with angiogenesis, cancer, and neurodevelopment. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 98(Pt A):25-33
Caballero-Gallardo, K., Olivero-Verbel, J., Freeman, J.L. (2016) Toxicogenomics to Evaluate Endocrine Disrupting Effects of Environmental Chemicals Using the Zebrafish Model. Current genomics. 17:515-527
Horzmann, K.A., Freeman, J.L. (2016) Zebrafish Get Connected: Investigating Neurotransmission Targets and Alterations in Chemical Toxicity. Toxics. 4(3)
Wirbisky, S.E., Sepúlveda, M.S., Weber, G.J., Jannasch, A.S., Horzmann, K.A., Freeman, J.L. (2016) Embryonic atrazine exposure elicits alterations in genes associated with neuroendocrine function in adult male zebrafish. Toxicological sciences : an official journal of the Society of Toxicology. 153(1):149-64
Lee, J., Peterson, S.M., Freeman, J.L. (2016) Alzheimer's disease risk genes in wild-type adult zebrafish exhibit gender-specific expression changes during aging. Neurogenetics. 17(3):197-9
Lee, J., Freeman, J.L. (2016) Embryonic exposure to 10 μg L(-1) lead results in female-specific expression changes in genes associated with nervous system development and function and Alzheimer's disease in aged adult zebrafish brain. Metallomics : integrated biometal science. 8(6):589-96
Wirbisky, S.E., Weber, G.J., Sepúlveda, M.S., Lin, T.L., Jannasch, A.S., Freeman, J.L. (2016) An embryonic atrazine exposure results in reproductive dysfunction in adult zebrafish and morphological alterations in their offspring. Scientific Reports. 6:21337
Wirbisky, S.E., Damayanti, N.P., Mahapatra, C.T., Sepulveda, M.S., Irudayaraj, J., Freeman, J.L. (2016) Mitochondrial Dysfunction, Disruption of F-actin Polymerization, and Transcriptomic Alterations in Zebrafish Larvae Exposed to Trichloroethylene. Chemical Research in Toxicology. 29(2):169-79
Bault, Z.A., Peterson, S.M., Freeman, J.L. (2015) Directional and color preference in adult zebrafish: Implications in behavioral and learning assays in neurotoxicology studies. Journal of applied toxicology : JAT. 35(12):1502-10
Wirbisky, S.E., Weber, G.J., Sepúlveda, M.S., Xiao, C., Cannon, J.R., Freeman, J.L. (2015) Developmental origins of neurotransmitter and transcriptome alterations in adult female zebrafish exposed to atrazine during embryogenesis. Toxicology. 333:156-67
Wirbisky, S.E., Weber, G.J., Lee, J.W., Cannon, J.R., Freeman, J.L. (2014) Novel dose-dependent alterations in excitatory GABA during embryonic development associated with lead (Pb) neurotoxicity. Toxicology letters. 229(1):1-8
Freeman, J.L., Weber, G.J., Peterson, S.M., Nie, L.H. (2014) Embryonic ionizing radiation exposure results in expression alterations of genes associated with cardiovascular and neurological development, function, and disease and modified cardiovascular function in zebrafish. Frontiers in genetics. 5:268
Lee, J., Freeman, J.L. (2014) Zebrafish as a model for investigating developmental lead (Pb) neurotoxicity as a risk factor in adult neurodegenerative disease: A mini-review. Neurotoxicology. 43:57-64
Peterson, S.M., Zhang, J., and Freeman, J.L. (2013) Developmental reelin expression and time point-specific alterations from lead exposure in zebrafish. Neurotoxicology and teratology. 38C:53-60
Weber, G.J., Sepulveda, M.S., Peterson, S.M., Lewis, S.S., and Freeman, J.L. (2013) Transcriptome alterations following developmental atrazine exposure in zebrafish are associated with disruption of neuroendocrine and reproductive system function, cell cycle, and carcinogenesis. Toxicological sciences : an official journal of the Society of Toxicology. 132(2):458-466
Brown, K.H., Dobrinski, K.P., Lee, A.S., Gokcumen, O., Mills, R.E., Shi, X., Chong, W.W., Chen, J.Y., Yoo, P., David, S., Peterson, S.M., Raj, T., Choy, K.W., Stranger, B.E., Williamson, R.E., Zon, L.I., Freeman, J.L., and Lee, C. (2012) Extensive genetic diversity and substructuring among zebrafish strains revealed through copy number variant analysis. Proceedings of the National Academy of Sciences of the United States of America. 109(2):529-34
Zhang, J., Peterson, S.M., Weber, G.J., Zhu, X., Zheng, W., and Freeman, J.L. (2011) Decreased axonal density and altered expression profiles of axonal guidance genes underlying lead (Pb) neurodevelopmental toxicity at early embryonic stages in the zebrafish. Neurotoxicology and teratology. 33(6):715-20
Peterson, S.M., Zhang, J., Weber, G., and Freeman, J.L. (2011) Global Gene Expression Analysis Reveals Dynamic and Developmental Stage Dependent Enrichment of Lead (Pb)-Induced Neurological Gene Alterations. Environmental health perspectives. 119(5):615-622
Dobrinski, K.P., Brown, K.H., Freeman, J.L., and Lee, C. (2011) Molecular Cytogenetic Methodologies and a BAC Probe Panel Resource for Genomic Analyses in the Zebrafish. Methods in cell biology. 104:237-257
Peterson, S.M., and Freeman, J.L. (2009) Cancer Cytogenetics in the Zebrafish. Zebrafish. 6(4):355-360
Peterson, S.M., and Freeman, J.L. (2009) Global gene expression analysis using a zebrafish oligonucleotide microarray platform. Journal of visualized experiments : JoVE. (30)
Peterson, S.M., and Freeman, J.L. (2009) RNA isolation from embryonic zebrafish and cDNA synthesis for gene expression analysis. Journal of visualized experiments : JoVE. (30)
Freeman, J.L., Ceol, C., Feng, H., Langenau, D.M., Belair, C., Stern, H.M., Song, A., Paw, B.H., Look, A.T., Zhou, Y., Zon, L.I., and Lee, C. (2009) Construction and application of a zebrafish array comparative genomic hybridization platform. Genes, chromosomes & cancer. 48(2):155-170
Yoder, J.A., Cannon, J.P., Litman, R.T., Murphy, C., Freeman, J.L., and Litman, G.W. (2008) Evidence for a transposition event in a second NITR gene cluster in zebrafish. Immunogenetics. 60(5):257-265
Freeman, J.L., Adeniyi, A., Banerjee, R., Dallaire, S., Maguire, S.F., Chi, J., Ng, B.L., Zepeda, C., Scott, C.E., Humphray, S., Rogers, J., Zhou, Y., Zon, L.I., Carter, N.P., Yang, F., and Lee, C. (2007) Definition of the zebrafish genome using flow cytometry and cytogenetic mapping. BMC Genomics. 8(1):195
Shepard, J.L., Amatruda, J.F., Finkelstein, D., Ziai, J., Finley, K.R., Stern, H.M., Chiang, K., Hersey, C., Barut, B., Freeman, J.L., Lee, C., Glickman, J.N., Kutok, J.L., Aster, J.C., and Zon, L.I. (2007) A mutation in separase causes genome instability and increased susceptibility to epithelial cancer. Genes & Development. 21(1):55-59

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