PUBLICATION

Knockdown of transcription factor forkhead box O3 (FOXO3) suppresses erythroid differentiation in human cells and zebrafish

Authors
Wang, H., Li, Y., Wang, S., Zhang, Q., Zheng, J., Yang, Y., Qi, H., Qu, H., Zhang, Z., Liu, F., Fang, X.
ID
ZDB-PUB-150407-2
Date
2015
Source
Biochemical and Biophysical Research Communications   460(4): 923-30 (Journal)
Registered Authors
Liu, Feng, Wang, Hai
Keywords
FOXO3, erythroid differentiation, globin, zebrafish
Datasets
GEO:GSE66860
MeSH Terms
  • Animals
  • Base Sequence
  • Cell Differentiation/genetics*
  • Erythrocytes/cytology*
  • Female
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism
  • Forkhead Transcription Factors/physiology*
  • Gene Knockdown Techniques*
  • Humans
  • Male
  • Neoplasm Proteins/metabolism
  • RNA, Small Interfering/genetics
  • Transcriptome
  • Zebrafish
PubMed
25843800 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Our previous study on the dynamic transcriptomes activated during human erythropoiesis suggested that transcription factor forkhead box O3 (FOXO3) possibly plays a role in erythroid differentiation. Functional studies in human cell line TF-1 indicated that FOXO3 knockdown repressed erythropoietin (EPO)-induced erythroid differentiation by activating promoter region of B-cell translocation gene 1 (BTG1), thereby regulating its expression. In zebrafish, injection of foxo3b-specific morpholinos (foxo3b MO) resulted in reduced globin (hbae1 and hbbe2) and gata1 gene expression. Transcriptome analyses of erythroid lineage cells isolated from the control and foxo3b morphants revealed the dynamic regulation of foxo3b. Further study suggested that BTG1 is partially responsible for FOXO3 regulation in erythroid differentiation of TF-1 cells but is inconsequential in zebrafish. Taken together, we found that FOXO3 plays an important role in erythroid differentiation in both human TF-1 cells and zebrafish, but the mechanism underlying this regulation still remains unclear.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping