PUBLICATION
Zebrafish vitamin K epoxide reductases: expression in vivo, along extracellular matrix mineralization and under phylloquinone and warfarin in vitro exposure
- Authors
- Fernández, I., Vijayakumar, P., Marques, C., Cancela, M.L., Gavaia, P.J., Laizé, V.
- ID
- ZDB-PUB-150325-25
- Date
- 2015
- Source
- Fish physiology and biochemistry 41(3): 745-59 (Journal)
- Registered Authors
- Cancela, Leonor
- Keywords
- none
- MeSH Terms
-
- Analysis of Variance
- Animals
- Base Sequence
- Calcification, Physiologic/drug effects
- Cell Line
- Computational Biology
- DNA Primers/genetics
- Extracellular Matrix/metabolism*
- Gene Expression Regulation/drug effects
- Gene Expression Regulation/genetics*
- Larva/growth & development
- Larva/metabolism
- Molecular Sequence Data
- Real-Time Polymerase Chain Reaction/veterinary
- Sequence Alignment
- Vitamin K 1/pharmacology
- Vitamin K Epoxide Reductases/genetics*
- Vitamin K Epoxide Reductases/metabolism*
- Warfarin/pharmacology
- Zebrafish/genetics*
- Zebrafish/growth & development*
- PubMed
- 25792234 Full text @ Fish Physiol. Biochem.
Citation
Fernández, I., Vijayakumar, P., Marques, C., Cancela, M.L., Gavaia, P.J., Laizé, V. (2015) Zebrafish vitamin K epoxide reductases: expression in vivo, along extracellular matrix mineralization and under phylloquinone and warfarin in vitro exposure. Fish physiology and biochemistry. 41(3):745-59.
Abstract
Vitamin K (VK) acts as a cofactor driving the biological activation of VK-dependent proteins and conferring calcium-binding properties to them. As a result, VK is converted into VK epoxide, which must be recycled by VK epoxide reductases (Vkors) before it can be reused. Although VK has been shown to play a central role in fish development, particularly during skeletogenesis, pathways underlying VK actions are poorly understood, while good and reliable molecular markers for VK cycle/homeostasis are still lacking in fish. In the present work, expression of 2 zebrafish vkor genes was characterized along larval development and in adult tissues through qPCR analysis. Zebrafish cell line ZFB1 was used to evaluate in vitro regulation of vkors and other VK cycle-related genes during mineralization and upon 24 h exposure to 0.16 and 0.8 µM phylloquinone (VK1), 0.032 µM warfarin, or a combination of both molecules. Results showed that zebrafish vkors are differentially expressed during larval development, in adult tissues, and during cell differentiation/mineralization processes. Further, several VK cycle intermediates were differentially expressed in ZFB1 cells exposed to VK1 and/or warfarin. Present work provides data identifying different developmental stages and adult tissues where VK recycling is probably highly required, and shows how genes involved in VK cycle respond to VK nutritional status in skeletal cells. Expression of vkor genes can represent a reliable indicator to infer VK nutritional status in fish, while ZFB1 cells could represent a suitable in vitro tool to get insights into the mechanisms underlying VK action on fish bone.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping