PUBLICATION

An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations

Authors
Burrows, J.T., Pearson, B.J., Scott, I.C.
ID
ZDB-PUB-150317-4
Date
2015
Source
Stem Cell Reports   4(4): 670-84 (Journal)
Registered Authors
Burrows, Jeff, Pearson, Bret, Scott, Ian
Keywords
none
Datasets
GEO:GSE58042
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/genetics
  • Cell Self Renewal/genetics*
  • Gene Expression
  • Immunohistochemistry
  • Mediator Complex/chemistry
  • Mediator Complex/genetics*
  • Mediator Complex/metabolism*
  • Mutation
  • Phenotype
  • Protein Subunits/genetics*
  • Protein Subunits/metabolism*
  • Stem Cells/cytology*
  • Stem Cells/metabolism*
  • Zebrafish
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed
25772472 Full text @ Stem Cell Reports
Abstract
The Mediator complex has recently been shown to be a key player in the maintenance of embryonic and induced pluripotent stem cells. However, the in vivo consequences of loss of many Mediator subunits are unknown. We identified med14 as the gene affected in the zebrafish logelei (log) mutant, which displayed a morphological arrest by 2 days of development. Surprisingly, microarray analysis showed that transcription was not broadly affected in log mutants. Indeed, log cells transplanted into a wild-type environment were able to survive into adulthood. In planarians, RNAi knockdown demonstrated a requirement for med14 and many other Mediator components in adult stem cell maintenance and regeneration. Multiple stem/progenitor cell populations were observed to be reduced or absent in zebrafish med14 mutant embryos. Taken together, our results show a critical, evolutionarily conserved, in vivo function for Med14 (and Mediator) in stem cell maintenance, distinct from a general role in transcription.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes