PUBLICATION

Identification of the optic recess region as a morphogenetic entity in the zebrafish forebrain

Authors

Affaticati, P., Yamamoto, K., Rizzi, B., Bureau, C., Peyriéras, N., Pasqualini, C., Demarque, M., Vernier, P.

ID

ZDB-PUB-150305-2

Date

2015

Source

Scientific Reports 5: 8738 (Journal)

Registered Authors

Demarque, Michael, Pasqualini, Catherine, Peyriéras, Nadine, Rizzi, Barbara, Vernier, Philippe, Yamamoto, Kei

Keywords

none

MeSH Terms

Models, Genetic
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Repressor Proteins/genetics
Repressor Proteins/metabolism
Animals
Models, Anatomic
ELAV-Like Protein 3/genetics
ELAV-Like Protein 3/metabolism
Neurogenesis/genetics*
Time Factors
Prosencephalon/embryology
Prosencephalon/metabolism*
Microscopy, Confocal
Zebrafish/embryology
Zebrafish/genetics*
Zebrafish/metabolism
In Situ Hybridization, Fluorescence
Reverse Transcriptase Polymerase Chain Reaction
Basic Helix-Loop-Helix Transcription Factors/genetics
Basic Helix-Loop-Helix Transcription Factors/metabolism
Embryo, Nonmammalian/anatomy & histology
Embryo, Nonmammalian/embryology
Embryo, Nonmammalian/metabolism
Preoptic Area/embryology
Preoptic Area/metabolism*
Gene Expression Regulation, Developmental

(all 27)

PubMed

25736911 Full text @ Sci. Rep.

Abstract

Regionalization is a critical, highly conserved step in the development of the vertebrate brain. Discrepancies exist in how regionalization of the anterior vertebrate forebrain is conceived since the "preoptic area" is proposed to be a part of the telencephalon in tetrapods but not in teleost fish. To gain insight into this complex morphogenesis, formation of the anterior forebrain was analyzed in 3D over time in zebrafish embryos, combining visualization of proliferation and differentiation markers, with that of developmental genes. We found that the region containing the preoptic area behaves as a coherent morphogenetic entity, organized around the optic recess and located between telencephalon and hypothalamus. This optic recess region (ORR) makes clear borders with its neighbor areas and expresses a specific set of genes (dlx2a, sim1a and otpb). We thus propose that the anterior forebrain (secondary prosencephalon) in teleosts contains three morphogenetic entities (telencephalon, ORR and hypothalamus), instead of two (telencephalon and hypothalamus). The ORR in teleosts could correspond to "telencephalic stalk area" and "alar hypothalamus" in tetrapods, resolving current inconsistencies in the comparison of basal forebrain among vertebrates.

Genes / Markers

Figures