PUBLICATION
Cis-regulatory control of human GLI2 expression in the developing neural tube and limb bud
- Authors
- Minhas, R., Pauls, S., Ali, S., Doglio, L., Khan, M.R., Elgar, G., Abbasi, A.A.
- ID
- ZDB-PUB-150227-10
- Date
- 2015
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 244(5): 681-92 (Journal)
- Registered Authors
- Doglio, Laura, Elgar, Greg, Minhas, Rashid, Pauls, Stefan
- Keywords
- CNE, GLI2, comparative genomics, enhancer, gene regulation, vertebrate development
- MeSH Terms
-
- Transcription Factors/biosynthesis*
- Transcription Factors/genetics
- Neural Tube/cytology
- Neural Tube/embryology*
- Humans
- Gene Expression Regulation, Developmental*
- Animals
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- Transcription, Genetic
- Nuclear Proteins/biosynthesis*
- Nuclear Proteins/genetics
- Limb Buds/cytology
- Limb Buds/embryology*
- Animals, Genetically Modified
- Kruppel-Like Transcription Factors/biosynthesis*
- Kruppel-Like Transcription Factors/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 25715918 Full text @ Dev. Dyn.
Citation
Minhas, R., Pauls, S., Ali, S., Doglio, L., Khan, M.R., Elgar, G., Abbasi, A.A. (2015) Cis-regulatory control of human GLI2 expression in the developing neural tube and limb bud. Developmental Dynamics : an official publication of the American Association of Anatomists. 244(5):681-92.
Abstract
Background GLI2, a zinc finger transcription factor, mediates Sonic hedgehog signaling, a critical pathway in vertebrate embryogenesis. GLI2 has been implicated in diverse set of embryonic developmental processes, including patterning of central nervous system and limbs. In humans, mutations in GLI2 are associated with several developmental defects, including holoprosencephaly and polydactyly. Results Here, we demonstrate in transient transgenic zebrafish assays, the potential of a subset of tetrapod-teleost conserved non-coding elements (CNEs) residing within human GLI2 intronic intervals to induce reporter gene expression at known regions of endogenous GLI2 transcription. The regulatory activities of these elements are observed in several embryonic domains, including neural tube and pectoral fin. Moreover, our data reveal an overlapping expression profile of duplicated copies of an enhancer during zebrafish evolution. Conclusions Our data suggest that during vertebrate history GLI2 acquired a high level of complexity in the genetic mechanisms regulating its expression during spatiotemporal patterning of the central nervous system (CNS) and limbs. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping