PUBLICATION
Primitive macrophages control HSPC mobilization and definitive haematopoiesis
- Authors
- Travnickova, J., Tran Chau, V., Julien, E., Mateos-Langerak, J., Gonzalez, C., Lelièvre, E., Lutfalla, G., Tavian, M., Kissa, K.
- ID
- ZDB-PUB-150218-6
- Date
- 2015
- Source
- Nature communications 6: 6227 (Journal)
- Registered Authors
- Kissa-Marin, Karima, Lutfalla, Georges
- Keywords
- none
- MeSH Terms
-
- Developmental Biology
- Zebrafish
- Extracellular Matrix/metabolism
- Microscopy, Fluorescence
- Macrophages/cytology*
- Macrophages/metabolism
- Matrix Metalloproteinase 9/metabolism
- Mesonephros/embryology
- Cell Lineage
- Matrix Metalloproteinase 13/metabolism
- Stem Cells/cytology*
- Animals
- Hematopoietic Stem Cells/cytology*
- Humans
- Aorta/embryology*
- Gonads/embryology
- Hematopoiesis*
- Animals, Genetically Modified
- PubMed
- 25686881 Full text @ Nat. Commun.
Citation
Travnickova, J., Tran Chau, V., Julien, E., Mateos-Langerak, J., Gonzalez, C., Lelièvre, E., Lutfalla, G., Tavian, M., Kissa, K. (2015) Primitive macrophages control HSPC mobilization and definitive haematopoiesis. Nature communications. 6:6227.
Abstract
In vertebrates, haematopoietic stem/progenitor cells (HSPCs) first emerge in the aorta-gonad-mesonephros (AGM) before colonizing transitory and subsequently definitive haematopoietic organs allowing haematopoiesis throughout adult life. Here we identify an unexpected primitive macrophage population accumulated in the dorsal mesenteric mesoderm surrounding the dorsal aorta of the human embryo and study its function in the transparent zebrafish embryo. Our study reveals dynamic interactions occurring between the HSPCs and primitive macrophages in the AGM. Specific chemical and inducible genetic depletion of macrophages or inhibition of matrix metalloproteinases (Mmps) leads to an accumulation of HSPCs in the AGM and a decrease in the colonization of haematopoietic organs. Finally, in vivo zymography demonstrates the function of primitive macrophages in extracellular matrix degradation, which allows HSPC migration through the AGM stroma, their intravasation, leading to the colonization of haematopoietic organs and the establishment of definitive haematopoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping