ZFIN ID: ZDB-PUB-150207-9
Two-Photon Fluorescent Probe for Detection of Exogenous and Endogenous Hydrogen Persulfide and Polysulfide in Living Organisms
Zeng, L., Chen, S., Xia, T., Hu, W., Li, C., Liu, Z.
Date: 2015
Source: Analytical chemistry   87(5): 3004-10 (Journal)
Registered Authors: Hu, Wei
Keywords: none
MeSH Terms:
  • Animals
  • Blotting, Western
  • Cystathionine beta-Synthase/metabolism
  • Cystathionine gamma-Lyase/metabolism
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism*
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen Sulfide/analysis*
  • Image Processing, Computer-Assisted/methods*
  • Photons*
  • Sulfides/analysis*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
PubMed: 25655109 Full text @ Anal. Chem.
ABSTRACT
Hydrogen persulfide and polysulfide (H2Sn) are the newly discovered intracellular reactive species considered to be with high protein S-sulfhydration efficiency. The detection of H2Sn in living systems is essential for studying their functions but quite challenging. In this work, we report a two-photon excited fluorescent probe, QSn, capable of tracing H2Sn in living organisms. QSn exhibited turn-on two-photon fluorescence response upon reaction with H2Sn. With favorable photophysical property, high specificity, and low cytotoxicity, QSn was able to recognize exogenous H2Sn in living cells. More importantly, it realized for the first time the visualization of endogenous H2Sn generated in cells overexpressing cystathionine β-synthase and cystathionine γ-lyase, the enzymes responsible for producing endogenous H2Sn. Taking advantages of two-photon microscopy, the probe was also applied to achieve H2Sn detection in zebrafish embryo and to observe the H2Sn distribution in living organisms.
ADDITIONAL INFORMATION No data available