Asymmetric inheritance of the apical domain and self-renewal of retinal ganglion cell progenitors depend on Anillin function

Paolini, A., Duchemin, A.L., Albadri, S., Patzel, E., Bornhorst, D., González Avalos, P., Lemke, S., Machate, A., Brand, M., Sel, S., Di Donato, V., Del Bene, F., Zolessi, F.R., Ramialison, M., Poggi, L.
Development (Cambridge, England)   142(5): 832-9 (Journal)
Registered Authors
Albadri, Shahad, Brand, Michael, Del Bene, Filippo, Duchemin, Anne-Laure, Machate, Anja, Paolini, Alessio, Patzel, Eva, Poggi, Lucia, Zolessi, Flavio
Anillin, Asymmetric cell division, Ath5 (Atoh7), Neurogenesis, Retina development, Apical domain inheritance
MeSH Terms
  • Animals
  • Contractile Proteins/genetics
  • Contractile Proteins/metabolism*
  • Fluorescent Antibody Technique
  • In Situ Hybridization
  • Microscopy, Confocal
  • Retinal Ganglion Cells/cytology*
  • Retinal Ganglion Cells/metabolism*
  • Stem Cells/cytology*
  • Stem Cells/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
25655700 Full text @ Development
Divisions that generate one neuronal lineage-committed and one self-renewing cell maintain the balance of proliferation and differentiation for the generation of neuronal diversity. The asymmetric inheritance of apical domains and components of the cell division machinery has been implicated in this process, and might involve interactions with cell fate determinants in regulatory feedback loops of an as yet unknown nature. Here, we report the dynamics of Anillin - an essential F-actin regulator and furrow component - and its contribution to progenitor cell divisions in the developing zebrafish retina. We find that asymmetrically dividing retinal ganglion cell progenitors position the Anillin-rich midbody at the apical domain of the differentiating daughter. anillin hypomorphic conditions disrupt asymmetric apical domain inheritance and affect daughter cell fate. Consequently, the retinal cell type composition is profoundly affected, such that the ganglion cell layer is dramatically expanded. This study provides the first in vivo evidence for the requirement of Anillin during asymmetric neurogenic divisions. It also provides insights into a reciprocal regulation between Anillin and the ganglion cell fate determinant Ath5, suggesting a mechanism whereby the balance of proliferation and differentiation is accomplished during progenitor cell divisions in vivo.
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes